Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases.

Published

Journal Article

Small ubiquitin-like modifier (SUMO1-3) is a small group of proteins that are ligated to lysine residues in target proteins. SUMO conjugation is a highly dynamic process, as SUMOylated proteins are rapidly deconjugated by SUMO proteases. SUMO conjugation/deconjugation plays pivotal roles in major cellular pathways and is associated with a number of pathological conditions. It is therefore of significant clinical interest to develop new strategies to screen for compounds to specifically interfere with SUMO conjugation/deconjugation. Here, we describe a novel high-throughput screening (HTS)-compatible assay to identify inhibitors of SUMO proteases. The assay is based on AlphaScreen technology and uses His-tagged SUMO2 conjugated to Strep-tagged SUMO3 as a SUMO protease substrate. A bacterial SUMOylation system was used to generate this substrate. A three-step purification strategy was employed to yield substrate of high quality. Our data indicated that this unique substrate can be readily detected in the AlphaScreen assays in a dose-dependent manner. Cleavage reactions by SUMO protease with or without inhibitor were monitored based on AlphaScreen signals. Furthermore, the assay was adapted to a 384-well format, and the interplate and interday variability was evaluated in eight 384-well plates. The average Z' factor was 0.83 ± 0.04, confirming the suitability for HTS applications.

Full Text

Duke Authors

Cited Authors

  • Yang, W; Wang, L; Paschen, W

Published Date

  • June 2013

Published In

Volume / Issue

  • 18 / 5

Start / End Page

  • 621 - 628

PubMed ID

  • 23470489

Pubmed Central ID

  • 23470489

Electronic International Standard Serial Number (EISSN)

  • 1552-454X

Digital Object Identifier (DOI)

  • 10.1177/1087057113479971

Language

  • eng

Conference Location

  • United States