Skip to main content

Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration.

Publication ,  Journal Article
Chen, Z; Lessey, E; Berginski, ME; Cao, L; Li, J; Trepat, X; Itano, M; Gomez, SM; Kapustina, M; Huang, C; Burridge, K; Truskey, G; Jacobson, K
Published in: PloS one
January 2013

The issue of how contractility and adhesion are related to cell shape and migration pattern remains largely unresolved. In this paper we report that Gleevec (Imatinib), an Abl family kinase inhibitor, produces a profound change in the shape and migration of rat bladder tumor cells (NBTII) plated on collagen-coated substrates. Cells treated with Gleevec adopt a highly spread D-shape and migrate more rapidly with greater persistence. Accompanying this more spread state is an increase in integrin-mediated adhesion coupled with increases in the size and number of discrete adhesions. In addition, both total internal reflection fluorescence microscopy (TIRFM) and interference reflection microscopy (IRM) revealed a band of small punctate adhesions with rapid turnover near the cell leading margin. These changes led to an increase in global cell-substrate adhesion strength, as assessed by laminar flow experiments. Gleevec-treated cells have greater RhoA activity which, via myosin activation, led to an increase in the magnitude of total traction force applied to the substrate. These chemical and physical alterations upon Gleevec treatment produce the dramatic change in morphology and migration that is observed.

Duke Scholars

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2013

Volume

8

Issue

1

Start / End Page

e52233

Related Subject Headings

  • rhoA GTP-Binding Protein
  • Urinary Bladder Neoplasms
  • Urinary Bladder
  • Tumor Cells, Cultured
  • Stress, Mechanical
  • Shear Strength
  • Rats
  • Pyrimidines
  • Proto-Oncogene Proteins c-abl
  • Piperazines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, Z., Lessey, E., Berginski, M. E., Cao, L., Li, J., Trepat, X., … Jacobson, K. (2013). Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration. PloS One, 8(1), e52233. https://doi.org/10.1371/journal.pone.0052233
Chen, Zaozao, Elizabeth Lessey, Matthew E. Berginski, Li Cao, Jonathan Li, Xavier Trepat, Michelle Itano, et al. “Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration.PloS One 8, no. 1 (January 2013): e52233. https://doi.org/10.1371/journal.pone.0052233.
Chen Z, Lessey E, Berginski ME, Cao L, Li J, Trepat X, et al. Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration. PloS one. 2013 Jan;8(1):e52233.
Chen, Zaozao, et al. “Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration.PloS One, vol. 8, no. 1, Jan. 2013, p. e52233. Epmc, doi:10.1371/journal.pone.0052233.
Chen Z, Lessey E, Berginski ME, Cao L, Li J, Trepat X, Itano M, Gomez SM, Kapustina M, Huang C, Burridge K, Truskey G, Jacobson K. Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration. PloS one. 2013 Jan;8(1):e52233.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2013

Volume

8

Issue

1

Start / End Page

e52233

Related Subject Headings

  • rhoA GTP-Binding Protein
  • Urinary Bladder Neoplasms
  • Urinary Bladder
  • Tumor Cells, Cultured
  • Stress, Mechanical
  • Shear Strength
  • Rats
  • Pyrimidines
  • Proto-Oncogene Proteins c-abl
  • Piperazines