Skip to main content
Journal cover image

Isolation of functional human endothelial cells from small volumes of umbilical cord blood.

Publication ,  Journal Article
Kang, SD; Carlon, TA; Jantzen, AE; Lin, F-H; Ley, MM; Allen, JD; Stabler, TV; Haley, NR; Truskey, GA; Achneck, HE
Published in: Annals of biomedical engineering
October 2013

Endothelial cells (ECs) isolated from endothelial progenitor cells in blood have great potential as a therapeutic tool to promote vasculogenesis and angiogenesis and treat cardiovascular diseases. However, current methods to isolate ECs are limited by a low yield with few colonies appearing during isolation. In order to utilize blood-derived ECs for therapeutic applications, a simple method is needed that can produce a high yield of ECs from small volumes of blood without the addition of animal-derived products. For the first time, we show that human ECs can be isolated without the prior separation of blood components through the technique of diluted whole blood incubation (DWBI) utilizing commercially available human serum. We isolated ECs from small volumes of blood (~10 mL) via DWBI and characterized them with flow cytometry, immunohistochemistry, and uptake of DiI-labeled acetylated low density lipoprotein (DiI-Ac-LDL). These ECs are functional as demonstrated by their ability to form tubular networks in Matrigel, adhere and align with flow under physiological fluid shear stress, and produce increased nitric oxide under fluid flow. An average of 7.0 ± 2.5 EC colonies that passed all functional tests described above were obtained per 10 mL of blood as compared to only 0.3 ± 0.1 colonies with the traditional method based on density centrifugation. The time until first colony appearance was 8.3 ± 1.2 days for ECs isolated with the DWBI method and 12 ± 1.4 days for ECs isolated with the traditional isolation method. A simplified method, such as DWBI, in combination with advances in isolation yield could enable the use of blood-derived ECs in clinical practice.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

October 2013

Volume

41

Issue

10

Start / End Page

2181 / 2192

Related Subject Headings

  • Time Factors
  • Male
  • Infant, Newborn
  • Humans
  • Flow Cytometry
  • Fetal Blood
  • Female
  • Endothelial Cells
  • Cells, Cultured
  • Biomedical Engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kang, S. D., Carlon, T. A., Jantzen, A. E., Lin, F.-H., Ley, M. M., Allen, J. D., … Achneck, H. E. (2013). Isolation of functional human endothelial cells from small volumes of umbilical cord blood. Annals of Biomedical Engineering, 41(10), 2181–2192. https://doi.org/10.1007/s10439-013-0807-5
Kang, Sa Do, Tim A. Carlon, Alexandra E. Jantzen, Fu-Hsiung Lin, Melissa M. Ley, Jason D. Allen, Thomas V. Stabler, N Rebecca Haley, George A. Truskey, and Hardean E. Achneck. “Isolation of functional human endothelial cells from small volumes of umbilical cord blood.Annals of Biomedical Engineering 41, no. 10 (October 2013): 2181–92. https://doi.org/10.1007/s10439-013-0807-5.
Kang SD, Carlon TA, Jantzen AE, Lin F-H, Ley MM, Allen JD, et al. Isolation of functional human endothelial cells from small volumes of umbilical cord blood. Annals of biomedical engineering. 2013 Oct;41(10):2181–92.
Kang, Sa Do, et al. “Isolation of functional human endothelial cells from small volumes of umbilical cord blood.Annals of Biomedical Engineering, vol. 41, no. 10, Oct. 2013, pp. 2181–92. Epmc, doi:10.1007/s10439-013-0807-5.
Kang SD, Carlon TA, Jantzen AE, Lin F-H, Ley MM, Allen JD, Stabler TV, Haley NR, Truskey GA, Achneck HE. Isolation of functional human endothelial cells from small volumes of umbilical cord blood. Annals of biomedical engineering. 2013 Oct;41(10):2181–2192.
Journal cover image

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

October 2013

Volume

41

Issue

10

Start / End Page

2181 / 2192

Related Subject Headings

  • Time Factors
  • Male
  • Infant, Newborn
  • Humans
  • Flow Cytometry
  • Fetal Blood
  • Female
  • Endothelial Cells
  • Cells, Cultured
  • Biomedical Engineering