Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.

Journal Article (Journal Article)

The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass approximately 400,000. Incorporation of the ryanodine receptor into lipid bilayers induced single Ca2+ channel currents with conductance and kinetic behavior almost identical to that of native cardiac Ca2+ release channels. These results suggest that the cardiac ryanodine receptor comprises the Ca2+ release channel involved in excitation-contraction coupling in cardiac muscle.

Full Text

Duke Authors

Cited Authors

  • Lai, FA; Anderson, K; Rousseau, E; Liu, QY; Meissner, G

Published Date

  • February 29, 1988

Published In

Volume / Issue

  • 151 / 1

Start / End Page

  • 441 - 449

PubMed ID

  • 2450540

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(88)90613-4


  • eng

Conference Location

  • United States