Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.
Journal Article (Journal Article)
The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass approximately 400,000. Incorporation of the ryanodine receptor into lipid bilayers induced single Ca2+ channel currents with conductance and kinetic behavior almost identical to that of native cardiac Ca2+ release channels. These results suggest that the cardiac ryanodine receptor comprises the Ca2+ release channel involved in excitation-contraction coupling in cardiac muscle.
- Lai, FA; Anderson, K; Rousseau, E; Liu, QY; Meissner, G
- February 29, 1988
Volume / Issue
- 151 / 1
Start / End Page
- 441 - 449
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States