A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma.
Journal Article (Journal Article)
Cysmethynil, a newly identified small molecule inhibitor of isoprenylcysteine carboxylmethyl transferase (Icmt) is involved in the post-translational modification of CaaX proteins. Cysmethynil causes cell death in many human cancer cells in vitro, and inhibits tumor growth in the xenograft mouse model in vivo. A HPLC method for the quantification of cysmethynil in mouse plasma was developed and validated. The lower limit of quantification of this method was 0.01microg/ml. Inter- and intra-day variability ranged from 0.38-8.5% and accuracy was between 86% and 98%. This sensitive method was used to quantify cysmethynil in plasma of mice after intraperitoneal dosing for preliminary pharmacokinetic studies.
Full Text
Duke Authors
Cited Authors
- Wang, M; Khoo, YM; Zhou, J; Casey, P; Lee, HS
Published Date
- February 15, 2009
Published In
Volume / Issue
- 877 / 5-6
Start / End Page
- 553 - 557
PubMed ID
- 19157999
International Standard Serial Number (ISSN)
- 1570-0232
Digital Object Identifier (DOI)
- 10.1016/j.jchromb.2008.12.067
Language
- eng
Conference Location
- Netherlands