A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma.

Journal Article (Journal Article)

Cysmethynil, a newly identified small molecule inhibitor of isoprenylcysteine carboxylmethyl transferase (Icmt) is involved in the post-translational modification of CaaX proteins. Cysmethynil causes cell death in many human cancer cells in vitro, and inhibits tumor growth in the xenograft mouse model in vivo. A HPLC method for the quantification of cysmethynil in mouse plasma was developed and validated. The lower limit of quantification of this method was 0.01microg/ml. Inter- and intra-day variability ranged from 0.38-8.5% and accuracy was between 86% and 98%. This sensitive method was used to quantify cysmethynil in plasma of mice after intraperitoneal dosing for preliminary pharmacokinetic studies.

Full Text

Duke Authors

Cited Authors

  • Wang, M; Khoo, YM; Zhou, J; Casey, P; Lee, HS

Published Date

  • February 15, 2009

Published In

Volume / Issue

  • 877 / 5-6

Start / End Page

  • 553 - 557

PubMed ID

  • 19157999

International Standard Serial Number (ISSN)

  • 1570-0232

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2008.12.067


  • eng

Conference Location

  • Netherlands