The differentiated form of nasopharyngeal carcinoma contains Epstein-Barr virus DNA.

Published

Journal Article

Immunologic studies of Epstein-Barr virus (EBV) have implicated EBV in undifferentiated and partially differentiated, non-keratinizing nasopharyngeal carcinoma (NPC). Patients with the well-differentiated, keratinizing form of NPC have EBV serologic patterns similar to those of control populations. In addition, viral DNA has not been detected in the differentiated tumors using viral cRNA probes to DNA immobilized on filters. In this study we have tested for EBV DNA using recombinant DNA probes to Southern blots of DNA from 33 NPC specimens. The 24 undifferentiated and 4 partially differentiated specimens generally contained a relatively high number of EBV genome equivalents, while the 5 well-differentiated NPC all contained detectable EBV, but at low copy number. The viral DNA from one of the well-differentiated specimens was cloned into a cosmid vector. Five recombinant clones representing the fused viral termini were obtained, indicating the presence of episomal, intracellular DNA in the tumor. These findings indicate that all histologic subsets of NPC contain EBV DNA.

Full Text

Duke Authors

Cited Authors

  • Raab-Traub, N; Flynn, K; Pearson, G; Huang, A; Levine, P; Lanier, A; Pagano, J

Published Date

  • January 15, 1987

Published In

Volume / Issue

  • 39 / 1

Start / End Page

  • 25 - 29

PubMed ID

  • 3025109

Pubmed Central ID

  • 3025109

International Standard Serial Number (ISSN)

  • 0020-7136

Digital Object Identifier (DOI)

  • 10.1002/ijc.2910390106

Language

  • eng

Conference Location

  • United States