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Further evidence that transposition of Tn5 in Escherichia coli is strongly enhanced by constitutively activated RecA proteins.

Publication ,  Journal Article
Kuan, CT; Tessman, I
Published in: J Bacteriol
November 1992

We have shown that excision and transposition of Tn5 in Escherichia coli are greatly increased by recA(Prtc) genes, which encode constitutively activated RecA proteins (C.-T. Kuan, S.-K. Liu, and I. Tessman, Genetics 128:45-57, 1991). Contrary results, showing a significant decrease in Tn5 transposition under SOS conditions, were subsequently reported (M. D. Weinreich, J. C. Makris, and W. S. Reznikoff, J. Bacteriol. 173:6910-6918, 1991). We have extended our studies to examine the following: (i) transposition of Tn5 from sites in the phoA, phoB, proC, trpD, and ilvD genes; (ii) the effect of gene transcription; (iii) the comparative effect of dinD+ and dinD(Def) alleles; (iv) the use of a mating-out assay of transposition; (v) the effect of a recA(Prtc) allele located at the normal chromosomal site; and (vi) the effect at 41.5 degrees C of the recA441(Prtc) allele. The new results fully confirm our previous conclusions, including the fact that the frequency of Tn5 transposition under constitutive SOS conditions is site dependent.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

November 1992

Volume

174

Issue

21

Start / End Page

6872 / 6877

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Rec A Recombinases
  • Microbiology
  • Genes, Bacterial
  • F Factor
  • Escherichia coli
  • DNA Transposable Elements
  • Crosses, Genetic
  • Alleles
  • 32 Biomedical and clinical sciences
 

Citation

APA
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MLA
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Kuan, C. T., & Tessman, I. (1992). Further evidence that transposition of Tn5 in Escherichia coli is strongly enhanced by constitutively activated RecA proteins. J Bacteriol, 174(21), 6872–6877. https://doi.org/10.1128/jb.174.21.6872-6877.1992
Kuan, C. T., and I. Tessman. “Further evidence that transposition of Tn5 in Escherichia coli is strongly enhanced by constitutively activated RecA proteins.J Bacteriol 174, no. 21 (November 1992): 6872–77. https://doi.org/10.1128/jb.174.21.6872-6877.1992.
Kuan, C. T., and I. Tessman. “Further evidence that transposition of Tn5 in Escherichia coli is strongly enhanced by constitutively activated RecA proteins.J Bacteriol, vol. 174, no. 21, Nov. 1992, pp. 6872–77. Pubmed, doi:10.1128/jb.174.21.6872-6877.1992.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

November 1992

Volume

174

Issue

21

Start / End Page

6872 / 6877

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Rec A Recombinases
  • Microbiology
  • Genes, Bacterial
  • F Factor
  • Escherichia coli
  • DNA Transposable Elements
  • Crosses, Genetic
  • Alleles
  • 32 Biomedical and clinical sciences