Skip to main content

Excision and transposition of Tn5 as an SOS activity in Escherichia coli.

Publication ,  Journal Article
Kuan, CT; Liu, SK; Tessman, I
Published in: Genetics
May 1991

Excision and transposition of the Tn5 element in Escherichia coli ordinarily appear to occur by recA-independent mechanisms. However, recA(Prtc) genes, which encode RecA proteins that are constitutively activated to the protease state, greatly enhanced excision and transposition; both events appeared to occur concomitantly and without destruction of the donor DNA. The recombinase function of the RecA protein was not required. Transposition was accompanied by partial, and occasionally full, restoration of the functional integrity of the gene vacated by the excised Tn5. The stimulation of transposition was inhibited by an uncleavable LexA protein and was strongly enhanced by an additional role of the RecA(Prtc) protein besides its mediation of LexA cleavage. To account for the enhanced transposition, we suggest that (i) there may be a LexA binding site within the promoter for the IS50 transposase, (ii) activated RecA may cleave the IS50 transposition inhibitor, and (iii) the transposase may be formed by RecA cleavage of a precursor molecule.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

May 1991

Volume

128

Issue

1

Start / End Page

45 / 57

Location

United States

Related Subject Headings

  • Serine Endopeptidases
  • SOS Response, Genetics
  • Restriction Mapping
  • Repressor Proteins
  • Rec A Recombinases
  • Molecular Sequence Data
  • Genes, Bacterial
  • Escherichia coli
  • Developmental Biology
  • DNA, Bacterial
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kuan, C. T., Liu, S. K., & Tessman, I. (1991). Excision and transposition of Tn5 as an SOS activity in Escherichia coli. Genetics, 128(1), 45–57. https://doi.org/10.1093/genetics/128.1.45
Kuan, C. T., S. K. Liu, and I. Tessman. “Excision and transposition of Tn5 as an SOS activity in Escherichia coli.Genetics 128, no. 1 (May 1991): 45–57. https://doi.org/10.1093/genetics/128.1.45.
Kuan CT, Liu SK, Tessman I. Excision and transposition of Tn5 as an SOS activity in Escherichia coli. Genetics. 1991 May;128(1):45–57.
Kuan, C. T., et al. “Excision and transposition of Tn5 as an SOS activity in Escherichia coli.Genetics, vol. 128, no. 1, May 1991, pp. 45–57. Pubmed, doi:10.1093/genetics/128.1.45.
Kuan CT, Liu SK, Tessman I. Excision and transposition of Tn5 as an SOS activity in Escherichia coli. Genetics. 1991 May;128(1):45–57.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

May 1991

Volume

128

Issue

1

Start / End Page

45 / 57

Location

United States

Related Subject Headings

  • Serine Endopeptidases
  • SOS Response, Genetics
  • Restriction Mapping
  • Repressor Proteins
  • Rec A Recombinases
  • Molecular Sequence Data
  • Genes, Bacterial
  • Escherichia coli
  • Developmental Biology
  • DNA, Bacterial