The in-vivo oxyhaemoglobin dissociation curve at sea level and high altitude.

Journal Article (Journal Article)

Animals native to hypoxic environments have adapted by increasing their haemoglobin oxygen affinity, but in-vitro studies of the oxyhaemoglobin dissociation curve (ODC) in humans show no changes in affinity under physiological conditions at altitudes up to 4000m. We conducted the first in-vivo measurement of the ODC; inducing progressive isocapnic hypoxia in lowlanders at sea level, acutely acclimatized lowlanders at 3600m, and native Andeans at that altitude. ODC curves were determined by administering isocapnic steps of increasing hypoxia, and measuring blood oxygen partial pressure and saturation. The ODC data were fitted using the Hill equation and extrapolated to predict the oxygen partial pressure at which haemoglobin was 50% saturated (P50). In contrast to findings from in-vitro studies, we found a pH-related reduction in P50 in subjects at altitude, compared to sea-level subjects. We conclude that a pH-mediated increase in haemoglobin oxygen affinity in-vivo may be part of the acclimatization process in humans at altitude.

Full Text

Duke Authors

Cited Authors

  • Balaban, DY; Duffin, J; Preiss, D; Mardimae, A; Vesely, A; Slessarev, M; Zubieta-Calleja, GR; Greene, ER; Macleod, DB; Fisher, JA

Published Date

  • March 1, 2013

Published In

Volume / Issue

  • 186 / 1

Start / End Page

  • 45 - 52

PubMed ID

  • 23313855

Electronic International Standard Serial Number (EISSN)

  • 1878-1519

Digital Object Identifier (DOI)

  • 10.1016/j.resp.2012.12.011


  • eng

Conference Location

  • Netherlands