Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family.

Journal Article

This paper describes the isolation and sequence of a human cDNA homologous to a class of proteins commonly referred to as 90-kDa heat-shock proteins. The complete nucleotide sequence of 2563 bp and the deduced amino acid sequence are presented. A single long open reading frame encodes a protein of 83,303 Da, the amino acid composition of which correlates well with that determined for the human 90-kDa heat-shock or 'stress' protein [Welch, W.J. and Feramisco, J.R., J. Biol. Chem. 257 (1982) 14949-14959]. Moreover, sequence analysis of this gene reveals extensive homology with the Drosophila 83-kDa and yeast 90-kDa heat-shock proteins. A comparison of the translated product of the human cDNA to the published yeast 90-kDa heat-shock protein reveals more than 60% homology at both the nucleotide and amino acid levels. Several regions of 50 aa or more show greater than 90% identity. This cDNA also hybridizes with an RNA species which increases upon heat shock of HeLa cells.

Full Text

Duke Authors

Cited Authors

  • Rebbe, NF; Ware, J; Bertina, RM; Modrich, P; Stafford, DW

Published Date

  • 1987

Published In

Volume / Issue

  • 53 / 2-3

Start / End Page

  • 235 - 245

PubMed ID

  • 3301534

Pubmed Central ID

  • 3301534

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(87)90012-6


  • eng

Conference Location

  • Netherlands