Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family.
Journal Article
This paper describes the isolation and sequence of a human cDNA homologous to a class of proteins commonly referred to as 90-kDa heat-shock proteins. The complete nucleotide sequence of 2563 bp and the deduced amino acid sequence are presented. A single long open reading frame encodes a protein of 83,303 Da, the amino acid composition of which correlates well with that determined for the human 90-kDa heat-shock or 'stress' protein [Welch, W.J. and Feramisco, J.R., J. Biol. Chem. 257 (1982) 14949-14959]. Moreover, sequence analysis of this gene reveals extensive homology with the Drosophila 83-kDa and yeast 90-kDa heat-shock proteins. A comparison of the translated product of the human cDNA to the published yeast 90-kDa heat-shock protein reveals more than 60% homology at both the nucleotide and amino acid levels. Several regions of 50 aa or more show greater than 90% identity. This cDNA also hybridizes with an RNA species which increases upon heat shock of HeLa cells.
Full Text
Duke Authors
Cited Authors
- Rebbe, NF; Ware, J; Bertina, RM; Modrich, P; Stafford, DW
Published Date
- 1987
Published In
Volume / Issue
- 53 / 2-3
Start / End Page
- 235 - 245
PubMed ID
- 3301534
Pubmed Central ID
- 3301534
International Standard Serial Number (ISSN)
- 0378-1119
Digital Object Identifier (DOI)
- 10.1016/0378-1119(87)90012-6
Language
- eng
Conference Location
- Netherlands