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Cytokinins can act as suppressors of nitric oxide in Arabidopsis.

Publication ,  Journal Article
Liu, W-Z; Kong, D-D; Gu, X-X; Gao, H-B; Wang, J-Z; Xia, M; Gao, Q; Tian, L-L; Xu, Z-H; Bao, F; Hu, Y; Ye, N-S; Pei, Z-M; He, Y-K
Published in: Proceedings of the National Academy of Sciences of the United States of America
January 2013

Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However, very little is known about the mechanisms of NO modulation in plants. Here, we report a unique mechanism for the catabolism of NO based on the reaction with the plant hormone cytokinin. We screened for NO-insensitive mutants in Arabidopsis and isolated two allelic lines, cnu1-1 and 1-2 (continuous NO-unstressed 1), that were identified as the previously reported altered meristem program 1 (amp1) and as having elevated levels of cytokinins. A double mutant of cnu1-2 and nitric oxide overexpression 1 (nox1) reduced the severity of the phenotypes ascribed to excess NO levels as did treating the nox1 line with trans-zeatin, the predominant form of cytokinin in Arabidopsis. We further showed that peroxinitrite, an active NO derivative, can react with zeatin in vitro, which together with the results in vivo suggests that cytokinins suppress the action of NO most likely through direct interaction between them, leading to the reduction of endogenous NO levels. These results provide insights into NO signaling and regulation of its bioactivity in plants.

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

January 2013

Volume

110

Issue

4

Start / End Page

1548 / 1553

Related Subject Headings

  • Zeatin
  • RNA, Plant
  • RNA, Messenger
  • Phenotype
  • Peroxynitrous Acid
  • Nitroprusside
  • Nitric Oxide Donors
  • Nitric Oxide
  • Mutation
  • Genes, Plant
 

Citation

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Liu, W.-Z., Kong, D.-D., Gu, X.-X., Gao, H.-B., Wang, J.-Z., Xia, M., … He, Y.-K. (2013). Cytokinins can act as suppressors of nitric oxide in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 110(4), 1548–1553. https://doi.org/10.1073/pnas.1213235110
Liu, Wei-Zhong, Dong-Dong Kong, Xue-Xin Gu, Hong-Bo Gao, Jin-Zheng Wang, Min Xia, Qian Gao, et al. “Cytokinins can act as suppressors of nitric oxide in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America 110, no. 4 (January 2013): 1548–53. https://doi.org/10.1073/pnas.1213235110.
Liu W-Z, Kong D-D, Gu X-X, Gao H-B, Wang J-Z, Xia M, et al. Cytokinins can act as suppressors of nitric oxide in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2013 Jan;110(4):1548–53.
Liu, Wei-Zhong, et al. “Cytokinins can act as suppressors of nitric oxide in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 4, Jan. 2013, pp. 1548–53. Epmc, doi:10.1073/pnas.1213235110.
Liu W-Z, Kong D-D, Gu X-X, Gao H-B, Wang J-Z, Xia M, Gao Q, Tian L-L, Xu Z-H, Bao F, Hu Y, Ye N-S, Pei Z-M, He Y-K. Cytokinins can act as suppressors of nitric oxide in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2013 Jan;110(4):1548–1553.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

January 2013

Volume

110

Issue

4

Start / End Page

1548 / 1553

Related Subject Headings

  • Zeatin
  • RNA, Plant
  • RNA, Messenger
  • Phenotype
  • Peroxynitrous Acid
  • Nitroprusside
  • Nitric Oxide Donors
  • Nitric Oxide
  • Mutation
  • Genes, Plant