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Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei.

Publication ,  Journal Article
Sun, GW; Chen, Y; Liu, Y; Tan, G-YG; Ong, C; Tan, P; Gan, Y-H
Published in: Mol Microbiol
May 2010

A major and critical virulence determinant of many Gram-negative bacterial pathogens is the Type III Secretion Systems (T3SS). T3SS3 in Burkholderia pseudomallei is critical for bacterial virulence in mammalian infection models but its regulation is unknown. B. pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in Southeast Asia and northern Australia. While screening for bacterial transposon mutants with a defective T3SS function, we discovered a TetR family regulator (bspR) responsible for the control of T3SS3 gene expression. The bspR mutant exhibited significant virulence attenuation in mice. BspR acts through BprP, a novel transmembrane regulator located adjacent to the currently delineated T3SS3 region. BprP in turn regulates the expression of structural and secretion components of T3SS3 and the AraC family regulator bsaN. BsaN and BicA likely form a complex to regulate the expression of T3SS3 effectors and other regulators which in turn affect the expression of Type VI Secretion Systems (T6SS). The complete delineation of the bspR initiated T3SS regulatory cascade not only contributes to the understanding of B. pseudomallei pathogenesis but also provides an important example of how bacterial pathogens could co-opt and integrate various regulatory motifs to form a new regulatory network adapted for its own purposes.

Duke Scholars

Published In

Mol Microbiol

DOI

EISSN

1365-2958

Publication Date

May 2010

Volume

76

Issue

3

Start / End Page

677 / 689

Location

England

Related Subject Headings

  • Virulence Factors
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Melioidosis
  • Humans
  • Gene Expression Regulation, Bacterial
  • Female
  • Burkholderia pseudomallei
  • Bacterial Proteins
 

Citation

APA
Chicago
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MLA
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Sun, G. W., Chen, Y., Liu, Y., Tan, G.-Y., Ong, C., Tan, P., & Gan, Y.-H. (2010). Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol, 76(3), 677–689. https://doi.org/10.1111/j.1365-2958.2010.07124.x
Sun, Guang Wen, Yahua Chen, Yichun Liu, Gek-Yen Gladys Tan, Catherine Ong, Patrick Tan, and Yunn-Hwen Gan. “Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei.Mol Microbiol 76, no. 3 (May 2010): 677–89. https://doi.org/10.1111/j.1365-2958.2010.07124.x.
Sun GW, Chen Y, Liu Y, Tan G-YG, Ong C, Tan P, et al. Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 2010 May;76(3):677–89.
Sun, Guang Wen, et al. “Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei.Mol Microbiol, vol. 76, no. 3, May 2010, pp. 677–89. Pubmed, doi:10.1111/j.1365-2958.2010.07124.x.
Sun GW, Chen Y, Liu Y, Tan G-YG, Ong C, Tan P, Gan Y-H. Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 2010 May;76(3):677–689.
Journal cover image

Published In

Mol Microbiol

DOI

EISSN

1365-2958

Publication Date

May 2010

Volume

76

Issue

3

Start / End Page

677 / 689

Location

England

Related Subject Headings

  • Virulence Factors
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Melioidosis
  • Humans
  • Gene Expression Regulation, Bacterial
  • Female
  • Burkholderia pseudomallei
  • Bacterial Proteins