The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis.

Journal Article (Journal Article)

Natural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. Using whole-genome Bp microarrays, we examined patterns of gene presence and absence across 94 South East Asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the Bp K96243 reference genome was common to all the strains representing the Bp "core genome", comprising genes largely involved in essential functions (eg amino acid metabolism, protein translation). In contrast, 14% of the K96243 genome was variably present across the isolates. This Bp accessory genome encompassed multiple genomic islands (GIs), paralogous genes, and insertions/deletions, including three distinct lipopolysaccharide (LPS)-related gene clusters. Strikingly, strains recovered from cases of human melioidosis clustered on a tree based on accessory gene content, and were significantly more likely to harbor certain GIs compared to animal and environmental isolates. Consistent with the inference that the GIs may contribute to pathogenesis, experimental mutation of BPSS2053, a GI gene, reduced microbial adherence to human epithelial cells. Our results suggest that the Bp accessory genome is likely to play an important role in microbial adaptation and virulence.

Full Text

Duke Authors

Cited Authors

  • Sim, SH; Yu, Y; Lin, CH; Karuturi, RKM; Wuthiekanun, V; Tuanyok, A; Chua, HH; Ong, C; Paramalingam, SS; Tan, G; Tang, L; Lau, G; Ooi, EE; Woods, D; Feil, E; Peacock, SJ; Tan, P

Published Date

  • October 2008

Published In

Volume / Issue

  • 4 / 10

Start / End Page

  • e1000178 -

PubMed ID

  • 18927621

Pubmed Central ID

  • PMC2564834

Electronic International Standard Serial Number (EISSN)

  • 1553-7374

Digital Object Identifier (DOI)

  • 10.1371/journal.ppat.1000178

Language

  • eng

Conference Location

  • United States