Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions.

Journal Article (Journal Article)

We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories.

Full Text

Duke Authors

Cited Authors

  • Akao, T; Sano, M; Yamada, O; Akeno, T; Fujii, K; Goto, K; Ohashi-Kunihiro, S; Takase, K; Yasukawa-Watanabe, M; Yamaguchi, K; Kurihara, Y; Maruyama, J-I; Juvvadi, PR; Tanaka, A; Hata, Y; Koyama, Y; Yamaguchi, S; Kitamoto, N; Gomi, K; Abe, K; Takeuchi, M; Kobayashi, T; Horiuchi, H; Kitamoto, K; Kashiwagi, Y; Machida, M; Akita, O

Published Date

  • April 2007

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 47 - 57

PubMed ID

  • 17540709

Pubmed Central ID

  • PMC2779895

International Standard Serial Number (ISSN)

  • 1340-2838

Digital Object Identifier (DOI)

  • 10.1093/dnares/dsm008


  • eng

Conference Location

  • England