Genome sequencing and analysis of Aspergillus oryzae.


Journal Article

The genome of Aspergillus oryzae, a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. The ability to secrete large amounts of proteins and the development of a transformation system have facilitated the use of A. oryzae in modern biotechnology. Although both A. oryzae and Aspergillus flavus belong to the section Flavi of the subgenus Circumdati of Aspergillus, A. oryzae, unlike A. flavus, does not produce aflatoxin, and its long history of use in the food industry has proved its safety. Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans and Aspergillus fumigatus. Comparison of the three aspergilli species revealed the presence of syntenic blocks and A. oryzae-specific blocks (lacking synteny with A. nidulans and A. fumigatus) in a mosaic manner throughout the genome of A. oryzae. The blocks of A. oryzae-specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.

Full Text

Duke Authors

Cited Authors

  • Machida, M; Asai, K; Sano, M; Tanaka, T; Kumagai, T; Terai, G; Kusumoto, K-I; Arima, T; Akita, O; Kashiwagi, Y; Abe, K; Gomi, K; Horiuchi, H; Kitamoto, K; Kobayashi, T; Takeuchi, M; Denning, DW; Galagan, JE; Nierman, WC; Yu, J; Archer, DB; Bennett, JW; Bhatnagar, D; Cleveland, TE; Fedorova, ND; Gotoh, O; Horikawa, H; Hosoyama, A; Ichinomiya, M; Igarashi, R; Iwashita, K; Juvvadi, PR; Kato, M; Kato, Y; Kin, T; Kokubun, A; Maeda, H; Maeyama, N; Maruyama, J-I; Nagasaki, H; Nakajima, T; Oda, K; Okada, K; Paulsen, I; Sakamoto, K; Sawano, T; Takahashi, M; Takase, K; Terabayashi, Y; Wortman, JR; Yamada, O; Yamagata, Y; Anazawa, H; Hata, Y; Koide, Y; Komori, T; Koyama, Y; Minetoki, T; Suharnan, S; Tanaka, A; Isono, K; Kuhara, S; Ogasawara, N; Kikuchi, H

Published Date

  • December 22, 2005

Published In

Volume / Issue

  • 438 / 7071

Start / End Page

  • 1157 - 1161

PubMed ID

  • 16372010

Pubmed Central ID

  • 16372010

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

Digital Object Identifier (DOI)

  • 10.1038/nature04300


  • eng

Conference Location

  • England