Discovery of a novel superfamily of type III polyketide synthases in Aspergillus oryzae.


Journal Article

Identification of genes encoding type III polyketide synthase (PKS) superfamily members in the industrially useful filamentous fungus, Aspergillus oryzae, revealed that their distribution is not specific to plants or bacteria. Among other Aspergilli (Aspergillus nidulans and Aspergillus fumigatus), A. oryzae was unique in possessing four chalcone synthase (CHS)-like genes (csyA, csyB, csyC, and csyD). Expression of csyA, csyB, and csyD genes was confirmed by RT-PCR. Comparative genome analyses revealed single putative type III PKS in Neurospora crassa and Fusarium graminearum, two each in Magnaporthe grisea and Podospora anserina, and three in Phenarocheate chrysosporium, with a phylogenic distinction from bacteria and plants. Conservation of catalytic residues in the CHSs across species implicated enzymatically active nature of these newly discovered homologs.

Full Text

Duke Authors

Cited Authors

  • Seshime, Y; Juvvadi, PR; Fujii, I; Kitamoto, K

Published Date

  • May 27, 2005

Published In

Volume / Issue

  • 331 / 1

Start / End Page

  • 253 - 260

PubMed ID

  • 15845386

Pubmed Central ID

  • 15845386

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2005.03.160


  • eng

Conference Location

  • United States