Functional analysis of the calcineurin-encoding gene cnaA from Aspergillus oryzae: evidence for its putative role in stress adaptation.


Journal Article

The presence of putative STRE (stress response regulatory element) and HSF (heat-shock factor) transcription factor binding sites in the promoter region of the gene encoding calcineurin ( cnaA) from Aspergillus oryzae implicated a probable role for calcineurin in the stress response. The activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 M NaCl (2.6-fold), at alkaline pH 10.0 (2.9-fold) and at 37 degrees C (1.6-fold). The induction of cnaA antisense expression resulted in reduced calcineurin activity (1.4-fold) and caused a growth defect under the stress conditions. Induction of a strain overexpressing cnaA resulted in an increase in calcineurin activity under stress conditions, such as the presence of 1 M NaCl (73%), alkaline pH 10.0 (70%), and a temperature of 37 degrees C (50%), in addition to tolerance to FK506 (a specific inhibitor of calcineurin). While a role for calcineurin in hyphal growth is well recognized, the present study suggests that stress adaptation mechanisms in filamentous fungi involve calmodulin/calcineurin-mediated signal transduction pathways.

Full Text

Duke Authors

Cited Authors

  • Juvvadi, PR; Kuroki, Y; Arioka, M; Nakajima, H; Kitamoto, K

Published Date

  • June 2003

Published In

Volume / Issue

  • 179 / 6

Start / End Page

  • 416 - 422

PubMed ID

  • 12709783

Pubmed Central ID

  • 12709783

International Standard Serial Number (ISSN)

  • 0302-8933

Digital Object Identifier (DOI)

  • 10.1007/s00203-003-0546-3


  • eng

Conference Location

  • Germany