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Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.

Publication ,  Journal Article
Kinev, AV; Levering, V; Young, K; Ali-Osman, F; Truskey, GA; Dewhirst, MW; Il'yasova, D
Published in: Cancer Invest
June 2013

Identification of measurable nontransient responses to low-dose radiation in human primary cell cultures remains a problem. To this end, circulating endothelial colony-forming (progenitor) cells (ECFCs) were examined as an experimental model. ECFCs were isolated from three cord blood donors. Cells were positive for endothelial cell markers and remained highly proliferative after long-term cryopreservation. A single dose of X-ray radiation (0.06-0.38 Gy) inhibited ECFC culture growth. This effect was evident at 48 hours and persisted up to 72 hr postirradiation. Such protracted cytostatic response of ECFCs to low-dose radiation suggests that ECFC primary cultures can be used to study low-dose radiation effects.

Duke Scholars

Published In

Cancer Invest

DOI

EISSN

1532-4192

Publication Date

June 2013

Volume

31

Issue

5

Start / End Page

359 / 364

Location

England

Related Subject Headings

  • Stem Cells
  • Radiation Injuries
  • Primary Cell Culture
  • Oncology & Carcinogenesis
  • Models, Biological
  • Humans
  • Endothelial Cells
  • Cells, Cultured
  • Cell Proliferation
  • 3211 Oncology and carcinogenesis
 

Citation

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Kinev, A. V., Levering, V., Young, K., Ali-Osman, F., Truskey, G. A., Dewhirst, M. W., & Il’yasova, D. (2013). Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose. Cancer Invest, 31(5), 359–364. https://doi.org/10.3109/07357907.2013.789903
Kinev, Alexander V., Vrad Levering, Kenneth Young, Francis Ali-Osman, George A. Truskey, Mark W. Dewhirst, and Dora Il’yasova. “Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.Cancer Invest 31, no. 5 (June 2013): 359–64. https://doi.org/10.3109/07357907.2013.789903.
Kinev AV, Levering V, Young K, Ali-Osman F, Truskey GA, Dewhirst MW, et al. Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose. Cancer Invest. 2013 Jun;31(5):359–64.
Kinev, Alexander V., et al. “Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.Cancer Invest, vol. 31, no. 5, June 2013, pp. 359–64. Pubmed, doi:10.3109/07357907.2013.789903.
Kinev AV, Levering V, Young K, Ali-Osman F, Truskey GA, Dewhirst MW, Il’yasova D. Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose. Cancer Invest. 2013 Jun;31(5):359–364.

Published In

Cancer Invest

DOI

EISSN

1532-4192

Publication Date

June 2013

Volume

31

Issue

5

Start / End Page

359 / 364

Location

England

Related Subject Headings

  • Stem Cells
  • Radiation Injuries
  • Primary Cell Culture
  • Oncology & Carcinogenesis
  • Models, Biological
  • Humans
  • Endothelial Cells
  • Cells, Cultured
  • Cell Proliferation
  • 3211 Oncology and carcinogenesis