B cell responses to the 2011/12-influenza vaccine in the aged.

Journal Article (Journal Article)

Antibody and B cell responses to influenza A viruses were measured over a period of 2 months in 30 aged and 15 middle-aged individuals following vaccination with the 2011/12 trivalent inactivated influenza vaccine by micro-neutralization assays, ELISAs, ELISpot assays and cell surface staining with lineage-defining antibodies followed by multicolor flow cytometry. Both cohorts developed comparable antibody responses to the H3N2 virus of the vaccine while responses to the H1N1 virus were compromised in the aged. ELISpot assays of peripheral blood mononuclear cells (PBMCs) gave comparable results for the two cohorts. Analysis by flow cytometry upon staining of CD19+IgD-CD20- PBMCs with antibodies to CD27 and CD38 showed markedly reduced increases of such cells following vaccination in the aged. Additional analysis of cells from a subset of 10 younger and 10 aged individuals indicated that in the aged a portion of IgG producing cells lose expression of CD27 and reduce expression of CD38.

Full Text

Duke Authors

Cited Authors

  • Kurupati, RK; Kannan, S; Xiang, Z-Q; Doyle, S; Ratcliffe, S; Schmader, KE; Ertl, HCJ

Published Date

  • March 2013

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • 209 - 226

PubMed ID

  • 23674565

Pubmed Central ID

  • PMC3629292

Electronic International Standard Serial Number (EISSN)

  • 1945-4589

Digital Object Identifier (DOI)

  • 10.18632/aging.100541


  • eng

Conference Location

  • United States