Detection of differentially regulated genes in ischaemic equine intestinal mucosa.

Published

Journal Article

REASONS FOR PERFORMING STUDY:Colic is a serious disease syndrome in horses. Much of the mortality is associated with ischaemic-injured intestine during strangulating obstruction, yet there is limited understanding of the associated molecular events. Identification of differentially expressed genes during ischaemic injury should expand our understanding of colic and may lead to novel targeted therapeutic approaches in the future. OBJECTIVE:To isolate and identify differentially expressed genes in equine jejunum following a 2 h ischaemic event compared to normally perfused jejunum. METHODS:Suppressive subtractive hybridisation was used to clone genes that are differentially expressed in equine jejunum injured by 2 h of complete ischaemia as compared to time-matched control jejunal tissues. Expression of selected clones was further evaluated by northern blot analysis. RESULTS:Of the 384 clones selected, 157 were confirmed to possess cDNAs corresponding differentially expressed genes by dot blot analysis. Two genes, fatty acid binding protein 2 and calcium-activated chloride channel 4 were further confirmed to be differentially expressed by northern blot analysis. CONCLUSIONS:Suppressive subtractive hybridisation can be used to detect changes in expression of a broad array of genes, as confirmed by northern blot analysis of selected genes. POTENTIAL RELEVANCE:These initial results have identified a pool of equine intestinal epithelial genes that are differentially expressed following a 2 h ischaemic event. In particular, genes indicative of deranged metabolic activity and those potentially involved in early repair events were identified and may ultimately provide clues as to the nature of epithelial ischaemic injury in horses.

Full Text

Cited Authors

  • Tschetter, JR; Blikslager, AT; Little, D; Howard, RD; Woody, SL; Beex, LM; Crisman, MV

Published Date

  • July 2005

Published In

Volume / Issue

  • 37 / 4

Start / End Page

  • 319 - 324

PubMed ID

  • 16028620

Pubmed Central ID

  • 16028620

Electronic International Standard Serial Number (EISSN)

  • 2042-3306

International Standard Serial Number (ISSN)

  • 0425-1644

Digital Object Identifier (DOI)

  • 10.2746/0425164054529382

Language

  • eng