Targeted ANP32E mutant mice do not demonstrate obvious movement defects.

Published online

Journal Article

BACKGROUND: The ANP32 family of proteins have been implicated in neuronal function through biochemical and cellular biology studies in neurons, as well as by recent behavioural studies of a gene-trapped loss-of-function mutation of Anp32e in mice, particularly with respect to fine motor function. A second targeted allele of the Anp32e, however, did not appear to demonstrate neurological phenotypes. METHODOLOGY/PRINCIPAL FINDINGS: Using a stringently controlled cohort of ten-generation backcrossed, co-caged, sex-matched, littermate pairs, we assayed for potential motor defects in the targeted ANP32E-deficient mice. We found no phenotypic difference in any assays. CONCLUSION: Since it is unlikely that the gene-trap is a more complete loss-of-function, our results suggest that ANP32E has no appreciable effect on motor functions and that genetic background differences most likely account for the gene-trap phenomena.

Full Text

Duke Authors

Cited Authors

  • Wong, P; Leo, VI; Low, M; Mak, TW; Zhang, X; Reilly, PT

Published Date

  • 2013

Published In

Volume / Issue

  • 8 / 5

Start / End Page

  • e63815 -

PubMed ID

  • 23675506

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0063815

Language

  • eng

Conference Location

  • United States