VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations.

Published

Journal Article

Mutations in VCP cause multisystem degeneration impacting the nervous system, muscle, and/or bone. Patients may present with ALS, Parkinsonism, frontotemporal dementia, myopathy, Paget's disease, or a combination of these. The disease mechanism is unknown. We developed a Drosophila model of VCP mutation-dependent degeneration. The phenotype is reminiscent of PINK1 and parkin mutants, including a pronounced mitochondrial defect. Indeed, VCP interacts genetically with the PINK1/parkin pathway in vivo. Paradoxically, VCP complements PINK1 deficiency but not parkin deficiency. The basis of this paradox is resolved by mechanistic studies in vitro showing that VCP recruitment to damaged mitochondria requires Parkin-mediated ubiquitination of mitochondrial targets. VCP recruitment coincides temporally with mitochondrial fission, and VCP is required for proteasome-dependent degradation of Mitofusins in vitro and in vivo. Further, VCP and its adaptor Npl4/Ufd1 are required for clearance of damaged mitochondria via the PINK1/Parkin pathway, and this is impaired by pathogenic mutations in VCP.

Full Text

Duke Authors

Cited Authors

  • Kim, NC; Tresse, E; Kolaitis, R-M; Molliex, A; Thomas, RE; Alami, NH; Wang, B; Joshi, A; Smith, RB; Ritson, GP; Winborn, BJ; Moore, J; Lee, J-Y; Yao, T-P; Pallanck, L; Kundu, M; Taylor, JP

Published Date

  • April 10, 2013

Published In

Volume / Issue

  • 78 / 1

Start / End Page

  • 65 - 80

PubMed ID

  • 23498974

Pubmed Central ID

  • 23498974

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2013.02.029

Language

  • eng

Conference Location

  • United States