MicroRNA target site identification by integrating sequence and binding information.
Journal Article (Journal Article)
High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute proteins can pinpoint a microRNA (miRNA) target site within tens of bases but leaves the identity of the miRNA unresolved. A flexible computational framework, microMUMMIE, integrates sequence with cross-linking features and reliably identifies the miRNA family involved in each binding event. It considerably outperforms sequence-only approaches and quantifies the prevalence of noncanonical binding modes.
Full Text
Duke Authors
Cited Authors
- Majoros, WH; Lekprasert, P; Mukherjee, N; Skalsky, RL; Corcoran, DL; Cullen, BR; Ohler, U
Published Date
- July 2013
Published In
Volume / Issue
- 10 / 7
Start / End Page
- 630 - 633
PubMed ID
- 23708386
Pubmed Central ID
- PMC3818907
Electronic International Standard Serial Number (EISSN)
- 1548-7105
Digital Object Identifier (DOI)
- 10.1038/nmeth.2489
Language
- eng
Conference Location
- United States