Nanoscale scaffolding domains within the postsynaptic density concentrate synaptic AMPA receptors.

Published

Journal Article

Scaffolding molecules at the postsynaptic membrane form the foundation of excitatory synaptic transmission by establishing the architecture of the postsynaptic density (PSD), but the small size of the synapse has precluded measurement of PSD organization in live cells. We measured the internal structure of the PSD in live neurons at approximately 25 nm resolution using photoactivated localization microscopy (PALM). We found that four major PSD scaffold proteins were each organized in distinctive ∼80 nm ensembles able to undergo striking changes over time. Bidirectional PALM and single-molecule immunolabeling showed that dense nanodomains of PSD-95 were preferentially enriched in AMPA receptors more than NMDA receptors. Chronic suppression of activity triggered changes in PSD interior architecture that may help amplify synaptic plasticity. The observed clustered architecture of the PSD controlled the amplitude and variance of simulated postsynaptic currents, suggesting several ways in which PSD interior organization may regulate the strength and plasticity of neurotransmission.

Full Text

Cited Authors

  • MacGillavry, HD; Song, Y; Raghavachari, S; Blanpied, TA

Published Date

  • May 2013

Published In

Volume / Issue

  • 78 / 4

Start / End Page

  • 615 - 622

PubMed ID

  • 23719161

Pubmed Central ID

  • 23719161

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2013.03.009

Language

  • eng