Skip to main content

The mechanism of nucleotide excision repair-mediated UV-induced mutagenesis in nonproliferating cells.

Publication ,  Journal Article
Kozmin, SG; Jinks-Robertson, S
Published in: Genetics
March 2013

Following the irradiation of nondividing yeast cells with ultraviolet (UV) light, most induced mutations are inherited by both daughter cells, indicating that complementary changes are introduced into both strands of duplex DNA prior to replication. Early analyses demonstrated that such two-strand mutations depend on functional nucleotide excision repair (NER), but the molecular mechanism of this unique type of mutagenesis has not been further explored. In the experiments reported here, an ade2 adeX colony-color system was used to examine the genetic control of UV-induced mutagenesis in nondividing cultures of Saccharomyces cerevisiae. We confirmed a strong suppression of two-strand mutagenesis in NER-deficient backgrounds and demonstrated that neither mismatch repair nor interstrand crosslink repair affects the production of these mutations. By contrast, proteins involved in the error-prone bypass of DNA damage (Rev3, Rev1, PCNA, Rad18, Pol32, and Rad5) and in the early steps of the DNA-damage checkpoint response (Rad17, Mec3, Ddc1, Mec1, and Rad9) were required for the production of two-strand mutations. There was no involvement, however, for the Pol η translesion synthesis DNA polymerase, the Mms2-Ubc13 postreplication repair complex, downstream DNA-damage checkpoint factors (Rad53, Chk1, and Dun1), or the Exo1 exonuclease. Our data support models in which UV-induced mutagenesis in nondividing cells occurs during the Pol ζ-dependent filling of lesion-containing, NER-generated gaps. The requirement for specific DNA-damage checkpoint proteins suggests roles in recruiting and/or activating factors required to fill such gaps.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

March 2013

Volume

193

Issue

3

Start / End Page

803 / 817

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Mutation Rate
  • Mutagenesis
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Repair
  • DNA Mismatch Repair
  • DNA Damage
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kozmin, S. G., & Jinks-Robertson, S. (2013). The mechanism of nucleotide excision repair-mediated UV-induced mutagenesis in nonproliferating cells. Genetics, 193(3), 803–817. https://doi.org/10.1534/genetics.112.147421
Kozmin, Stanislav G., and Sue Jinks-Robertson. “The mechanism of nucleotide excision repair-mediated UV-induced mutagenesis in nonproliferating cells.Genetics 193, no. 3 (March 2013): 803–17. https://doi.org/10.1534/genetics.112.147421.
Kozmin, Stanislav G., and Sue Jinks-Robertson. “The mechanism of nucleotide excision repair-mediated UV-induced mutagenesis in nonproliferating cells.Genetics, vol. 193, no. 3, Mar. 2013, pp. 803–17. Pubmed, doi:10.1534/genetics.112.147421.

Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

March 2013

Volume

193

Issue

3

Start / End Page

803 / 817

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Mutation Rate
  • Mutagenesis
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Repair
  • DNA Mismatch Repair
  • DNA Damage