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Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent.

Publication ,  Journal Article
Bhavane, R; Badea, C; Ghaghada, KB; Clark, D; Vela, D; Moturu, A; Annapragada, A; Johnson, GA; Willerson, JT; Annapragada, A
Published in: Circ Cardiovasc Imaging
March 1, 2013

BACKGROUND: The accumulation of macrophages in inflamed atherosclerotic plaques has long been recognized. In an attempt to develop an imaging agent for detection of vulnerable plaques, we evaluated the feasibility of a liposomal-iodine nanoparticle contrast agent for computed tomography imaging of macrophage-rich atherosclerotic plaques in a mouse model. METHODS AND RESULTS: Liposomal-iodine formulations varying in particle size and polyethylene glycol coating were fabricated and shown to stably encapsulate the iodine compound. In vitro uptake studies using optical and computed tomography imaging in the RAW 264.7 macrophage cell line identified the formulation that promoted maximal uptake. Dual-energy computed tomography imaging using this formulation in apolipoprotein E-deficient (ApoE(-/-)) mice (n=8) and control C57BL/6 mice (n=6) followed by spectral decomposition of the dual-energy images enabled imaging of the liposomes localized in the plaque. Imaging cytometry confirmed the presence of liposomes in the plaque and their colocalization with a small fraction (≈2%) of the macrophages in the plaque. CONCLUSIONS: The results demonstrate the feasibility of imaging macrophage-rich atherosclerotic plaques using a liposomal-iodine nanoparticle contrast agent and dual-energy computed tomography.

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Published In

Circ Cardiovasc Imaging

DOI

EISSN

1942-0080

Publication Date

March 1, 2013

Volume

6

Issue

2

Start / End Page

285 / 294

Location

United States

Related Subject Headings

  • Triiodobenzoic Acids
  • Tomography, X-Ray Computed
  • Time Factors
  • Predictive Value of Tests
  • Plaque, Atherosclerotic
  • Nanoparticles
  • Microscopy, Fluorescence
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

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Bhavane, R., Badea, C., Ghaghada, K. B., Clark, D., Vela, D., Moturu, A., … Willerson, J. T. (2013). Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent. Circ Cardiovasc Imaging, 6(2), 285–294. https://doi.org/10.1161/CIRCIMAGING.112.000119
Bhavane, Rohan, Cristian Badea, Ketan B. Ghaghada, Darin Clark, Deborah Vela, Anoosha Moturu, Akshaya Annapragada, G Allan Johnson, James T. Willerson, and Ananth Annapragada. “Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent.Circ Cardiovasc Imaging 6, no. 2 (March 1, 2013): 285–94. https://doi.org/10.1161/CIRCIMAGING.112.000119.
Bhavane R, Badea C, Ghaghada KB, Clark D, Vela D, Moturu A, et al. Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent. Circ Cardiovasc Imaging. 2013 Mar 1;6(2):285–94.
Bhavane, Rohan, et al. “Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent.Circ Cardiovasc Imaging, vol. 6, no. 2, Mar. 2013, pp. 285–94. Pubmed, doi:10.1161/CIRCIMAGING.112.000119.
Bhavane R, Badea C, Ghaghada KB, Clark D, Vela D, Moturu A, Annapragada A, Johnson GA, Willerson JT. Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent. Circ Cardiovasc Imaging. 2013 Mar 1;6(2):285–294.

Published In

Circ Cardiovasc Imaging

DOI

EISSN

1942-0080

Publication Date

March 1, 2013

Volume

6

Issue

2

Start / End Page

285 / 294

Location

United States

Related Subject Headings

  • Triiodobenzoic Acids
  • Tomography, X-Ray Computed
  • Time Factors
  • Predictive Value of Tests
  • Plaque, Atherosclerotic
  • Nanoparticles
  • Microscopy, Fluorescence
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice