Assessment of gene expression in peripheral blood using RNAseq before and after weight restoration in anorexia nervosa.

Journal Article (Journal Article)

We examined gene expression in the blood of six females with anorexia nervosa (AN) before and after weight restoration using RNAseq. AN cases (aged 19-39) completed clinical assessments and had blood drawn for RNA at hospital admission (T1,<~75% ideal body weight, IBW) and again at discharge (T2,≥ ~ 85% IBW). To examine the relationship between weight restoration and differential gene expression, normalized gene expression levels were analyzed using a paired design. We found 564 genes whose expression was nominally significantly different following weight restoration (p<0.01, 231 increased and 333 decreased). With a more stringent significance threshold (false discovery rate q<0.05), 67 genes met criteria for differential expression. Of the top 20 genes, CYP11A1, C16orf11, LINC00235, and CPA3 were down-regulated more than two-fold after weight restoration while multiple olfactory receptor genes (OR52J3, OR51L1, OR51A4, and OR51A2) were up-regulated more than two-fold after weight restoration. Pathway analysis revealed up-regulation of two broad pathways with largely overlapping genes, one related to protein secretion and signaling and the other associated with defense response to bacterial regulation. Although results are preliminary secondary to a small sample size, these data provide initial evidence of transcriptional alterations during weight restoration in AN.

Full Text

Duke Authors

Cited Authors

  • Kim, Y; Trace, SE; Crowley, JJ; Brownley, KA; Hamer, RM; Pisetsky, DS; Sullivan, PF; Bulik, CM

Published Date

  • November 30, 2013

Published In

Volume / Issue

  • 210 / 1

Start / End Page

  • 287 - 293

PubMed ID

  • 23778302

Pubmed Central ID

  • PMC3805820

Electronic International Standard Serial Number (EISSN)

  • 1872-7123

Digital Object Identifier (DOI)

  • 10.1016/j.psychres.2013.05.026


  • eng

Conference Location

  • Ireland