Direct methods for detection of protein S-nitrosylation.


Journal Article (Review)

S-nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This chapter provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties.

Full Text

Cited Authors

  • Devarie-Baez, NO; Zhang, D; Li, S; Whorton, AR; Xian, M

Published Date

  • August 2013

Published In

Volume / Issue

  • 62 / 2

Start / End Page

  • 171 - 176

PubMed ID

  • 23639867

Pubmed Central ID

  • 23639867

Electronic International Standard Serial Number (EISSN)

  • 1095-9130

International Standard Serial Number (ISSN)

  • 1046-2023

Digital Object Identifier (DOI)

  • 10.1016/j.ymeth.2013.04.018


  • eng