Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.

Journal Article

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Full Text

Duke Authors

Cited Authors

  • Lyon, GM; Abdul-Ali, D; Loeffler, J; White, PL; Wickes, B; Herrera, ML; Alexander, BD; Baden, LR; Clancy, C; Denning, D; Nguyen, MH; Sugrue, M; Wheat, LJ; Wingard, JR; Donnelly, JP; Barnes, R; Patterson, TF; Caliendo, AM; AsTeC, IAAM, EAPCRI Investigators,

Published Date

  • July 2013

Published In

Volume / Issue

  • 51 / 7

Start / End Page

  • 2403 - 2405

PubMed ID

  • 23616459

Pubmed Central ID

  • 23616459

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.00744-13

Language

  • eng