Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.
Journal Article
Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.
Full Text
Duke Authors
Cited Authors
- Lyon, GM; Abdul-Ali, D; Loeffler, J; White, PL; Wickes, B; Herrera, ML; Alexander, BD; Baden, LR; Clancy, C; Denning, D; Nguyen, MH; Sugrue, M; Wheat, LJ; Wingard, JR; Donnelly, JP; Barnes, R; Patterson, TF; Caliendo, AM; AsTeC, IAAM, EAPCRI Investigators,
Published Date
- July 2013
Published In
Volume / Issue
- 51 / 7
Start / End Page
- 2403 - 2405
PubMed ID
- 23616459
Pubmed Central ID
- 23616459
Electronic International Standard Serial Number (EISSN)
- 1098-660X
International Standard Serial Number (ISSN)
- 0095-1137
Digital Object Identifier (DOI)
- 10.1128/jcm.00744-13
Language
- eng