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Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.

Publication ,  Journal Article
Lyon, GM; Abdul-Ali, D; Loeffler, J; White, PL; Wickes, B; Herrera, ML; Alexander, BD; Baden, LR; Clancy, C; Denning, D; Nguyen, MH; Sugrue, M ...
Published in: J Clin Microbiol
July 2013

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Duke Scholars

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

July 2013

Volume

51

Issue

7

Start / End Page

2403 / 2405

Location

United States

Related Subject Headings

  • Reference Standards
  • Molecular Diagnostic Techniques
  • Microbiology
  • Microbiological Techniques
  • Humans
  • DNA, Fungal
  • Aspergillus
  • Aspergillosis
  • 3207 Medical microbiology
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lyon, G. M., Abdul-Ali, D., Loeffler, J., White, P. L., Wickes, B., Herrera, M. L., … AsTeC, IAAM, EAPCRI Investigators, . (2013). Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis. J Clin Microbiol, 51(7), 2403–2405. https://doi.org/10.1128/JCM.00744-13
Lyon, G Marshall, Deborah Abdul-Ali, Juergen Loeffler, P Lewis White, Brian Wickes, Monica L. Herrera, Barbara D. Alexander, et al. “Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.J Clin Microbiol 51, no. 7 (July 2013): 2403–5. https://doi.org/10.1128/JCM.00744-13.
Lyon GM, Abdul-Ali D, Loeffler J, White PL, Wickes B, Herrera ML, et al. Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis. J Clin Microbiol. 2013 Jul;51(7):2403–5.
Lyon, G. Marshall, et al. “Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.J Clin Microbiol, vol. 51, no. 7, July 2013, pp. 2403–05. Pubmed, doi:10.1128/JCM.00744-13.
Lyon GM, Abdul-Ali D, Loeffler J, White PL, Wickes B, Herrera ML, Alexander BD, Baden LR, Clancy C, Denning D, Nguyen MH, Sugrue M, Wheat LJ, Wingard JR, Donnelly JP, Barnes R, Patterson TF, Caliendo AM, AsTeC, IAAM, EAPCRI Investigators. Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis. J Clin Microbiol. 2013 Jul;51(7):2403–2405.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

July 2013

Volume

51

Issue

7

Start / End Page

2403 / 2405

Location

United States

Related Subject Headings

  • Reference Standards
  • Molecular Diagnostic Techniques
  • Microbiology
  • Microbiological Techniques
  • Humans
  • DNA, Fungal
  • Aspergillus
  • Aspergillosis
  • 3207 Medical microbiology
  • 3202 Clinical sciences