Development of a method to isolate circulating tumor cells using mesenchymal-based capture.

Journal Article (Journal Article)

Epithelial tumor cells can become mesenchymal cells and vice versa via phenotypic transitions, a process known as epithelial plasticity. We postulate that during the process of metastasis, circulating tumor cells (CTCs) lose their epithelial phenotype and acquire a mesenchymal phenotype that may not be sufficiently captured by existing epithelial-based CTC technologies. We report here on the development of a novel CTC capture method, based on the biology of epithelial plasticity, which isolates cells based on OB-cadherin cell surface expression. Using this mesenchymal-based assay, OB-cadherin cellular events are detectable in men with metastatic prostate cancer and are less common in healthy volunteers. This method may complement existing epithelial-based methods and may be particularly useful in patients with bone metastases.

Full Text

Duke Authors

Cited Authors

  • Bitting, RL; Boominathan, R; Rao, C; Kemeny, G; Foulk, B; Garcia-Blanco, MA; Connelly, M; Armstrong, AJ

Published Date

  • December 1, 2013

Published In

Volume / Issue

  • 64 / 2

Start / End Page

  • 129 - 136

PubMed ID

  • 23845299

Pubmed Central ID

  • PMC3833983

Electronic International Standard Serial Number (EISSN)

  • 1095-9130

Digital Object Identifier (DOI)

  • 10.1016/j.ymeth.2013.06.034


  • eng

Conference Location

  • United States