Optical clearing and multiphoton imaging of paraffin-embedded specimens

Conference Paper

New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffinembedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies. © 2013 SPIE.

Full Text

Duke Authors

Cited Authors

  • Wilson, JW; Degan, S; Fischer, MC; Warren, WS

Published Date

  • June 12, 2013

Published In

Volume / Issue

  • 8588 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9780819493576

Digital Object Identifier (DOI)

  • 10.1117/12.2003155

Citation Source

  • Scopus