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Comparison of intradermal and intramuscular delivery followed by in vivo electroporation of SIV Env DNA in macaques.

Publication ,  Journal Article
Kulkarni, V; Rosati, M; Bear, J; Pilkington, GR; Jalah, R; Bergamaschi, C; Singh, AK; Alicea, C; Chowdhury, B; Zhang, G-M; Kim, E-Y; Huang, W ...
Published in: Hum Vaccin Immunother
October 2013

A panel of SIVmac251 transmitted Env sequences were tested for expression, function and immunogenicity in mice and macaques. The immunogenicity of a DNA vaccine cocktail expressing SIVmac239 and three transmitted SIVmac251 Env sequences was evaluated upon intradermal or intramuscular injection followed by in vivo electroporation in macaques using sequential vaccination of gp160, gp120 and gp140 expressing DNAs. Both intradermal and intramuscular vaccination regimens using the gp160 expression plasmids induced robust humoral immune responses, which further improved using the gp120 expressing DNAs. The responses showed durability of binding and neutralizing antibody titers and high avidity for>1 y. The intradermal DNA delivery regimen induced higher cross-reactive responses able to neutralize the heterologous tier 1B-like SIVsmE660_CG7V. Analysis of cellular immune responses showed induction of Env-specific memory responses and cytotoxic granzyme B(+) T cells in both vaccine groups, although the magnitude of the responses were ~10x higher in the intramuscular/electroporation group. The cellular responses induced by both regimens were long lasting and could be detected ~1 y after the last vaccination. These data show that both DNA delivery methods are able to induce robust and durable immune responses in macaques.

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Published In

Hum Vaccin Immunother

DOI

EISSN

2164-554X

Publication Date

October 2013

Volume

9

Issue

10

Start / End Page

2081 / 2094

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Vaccination
  • Time Factors
  • T-Lymphocytes, Cytotoxic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
  • Mice, Inbred BALB C
  • Mice
 

Citation

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Chicago
ICMJE
MLA
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Kulkarni, V., Rosati, M., Bear, J., Pilkington, G. R., Jalah, R., Bergamaschi, C., … Pavlakis, G. N. (2013). Comparison of intradermal and intramuscular delivery followed by in vivo electroporation of SIV Env DNA in macaques. Hum Vaccin Immunother, 9(10), 2081–2094. https://doi.org/10.4161/hv.25473
Kulkarni, Viraj, Margherita Rosati, Jenifer Bear, Guy R. Pilkington, Rashmi Jalah, Cristina Bergamaschi, Ashish K. Singh, et al. “Comparison of intradermal and intramuscular delivery followed by in vivo electroporation of SIV Env DNA in macaques.Hum Vaccin Immunother 9, no. 10 (October 2013): 2081–94. https://doi.org/10.4161/hv.25473.
Kulkarni V, Rosati M, Bear J, Pilkington GR, Jalah R, Bergamaschi C, et al. Comparison of intradermal and intramuscular delivery followed by in vivo electroporation of SIV Env DNA in macaques. Hum Vaccin Immunother. 2013 Oct;9(10):2081–94.
Kulkarni, Viraj, et al. “Comparison of intradermal and intramuscular delivery followed by in vivo electroporation of SIV Env DNA in macaques.Hum Vaccin Immunother, vol. 9, no. 10, Oct. 2013, pp. 2081–94. Pubmed, doi:10.4161/hv.25473.
Kulkarni V, Rosati M, Bear J, Pilkington GR, Jalah R, Bergamaschi C, Singh AK, Alicea C, Chowdhury B, Zhang G-M, Kim E-Y, Wolinsky SM, Huang W, Guan Y, LaBranche C, Montefiori DC, Broderick KE, Sardesai NY, Valentin A, Felber BK, Pavlakis GN. Comparison of intradermal and intramuscular delivery followed by in vivo electroporation of SIV Env DNA in macaques. Hum Vaccin Immunother. 2013 Oct;9(10):2081–2094.

Published In

Hum Vaccin Immunother

DOI

EISSN

2164-554X

Publication Date

October 2013

Volume

9

Issue

10

Start / End Page

2081 / 2094

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Vaccination
  • Time Factors
  • T-Lymphocytes, Cytotoxic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
  • Mice, Inbred BALB C
  • Mice