Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution
Publication
, Journal Article
Simpson, MJ; Wilson, JW; Phipps, MA; Robles, FE; Selim, MA; Warren, WS
Published in: Journal of Investigative Dermatology
2013
Pump-probe microscopy nondestructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a subcellular scale, allowing elucidation of characteristics of different cell types. The results show that melanin heterogeneity, previously found to be characteristic of melanomas, persists on the subcellular scale. We have also found spectral changes associated with melanin located in melanophages that could potentially differentiate invasive pigmented melanocytes from melanophages without immunohistochemical staining. © 2013 The Society for Investigative Dermatology.
Duke Scholars
Published In
Journal of Investigative Dermatology
DOI
ISSN
0022-202X
Publication Date
2013
Volume
133
Issue
7
Start / End Page
1822 / 1826
Related Subject Headings
- Dermatology & Venereal Diseases
- 1112 Oncology and Carcinogenesis
- 1103 Clinical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Simpson, M. J., Wilson, J. W., Phipps, M. A., Robles, F. E., Selim, M. A., & Warren, W. S. (2013). Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. Journal of Investigative Dermatology, 133(7), 1822–1826. https://doi.org/10.1038/jid.2013.37
Simpson, M. J., J. W. Wilson, M. A. Phipps, F. E. Robles, M. A. Selim, and W. S. Warren. “Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution.” Journal of Investigative Dermatology 133, no. 7 (2013): 1822–26. https://doi.org/10.1038/jid.2013.37.
Simpson MJ, Wilson JW, Phipps MA, Robles FE, Selim MA, Warren WS. Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. Journal of Investigative Dermatology. 2013;133(7):1822–6.
Simpson, M. J., et al. “Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution.” Journal of Investigative Dermatology, vol. 133, no. 7, 2013, pp. 1822–26. Scival, doi:10.1038/jid.2013.37.
Simpson MJ, Wilson JW, Phipps MA, Robles FE, Selim MA, Warren WS. Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. Journal of Investigative Dermatology. 2013;133(7):1822–1826.
Published In
Journal of Investigative Dermatology
DOI
ISSN
0022-202X
Publication Date
2013
Volume
133
Issue
7
Start / End Page
1822 / 1826
Related Subject Headings
- Dermatology & Venereal Diseases
- 1112 Oncology and Carcinogenesis
- 1103 Clinical Sciences