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Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy.

Publication ,  Journal Article
Ma, J; Tian, L; Cheng, J; Chen, Z; Xu, B; Wang, L; Li, C; Huang, Q
Published in: PLoS One
2013

Tumor growth after radiotherapy is a commonly recognized cause of therapeutic failure. In this way, we examined tumor cell growth after radiotherapy by establishing a cancer cell growth model in vitro. We accomplished this model by seeding non-irradiated firefly luciferase2 and green fluorescent protein fusion gene (Fluc) labeled living cancer reporter cells onto a feeder layer of irradiated cancer cells. The living tumor cell growth was monitored by bioluminescence imaging. The living reporter cells grew faster when seeded onto lethally irradiated feeder cells than when seeded onto non-irradiated feeder cells or when seeded in the absence of feeder cells. We found that the expression levels of the Shh and Gli1 proteins, both of which are critical proteins in Sonic hedgehog (SHH) signaling, were increased after irradiation and that this expression was positively correlated with reporter cell growth. Moreover, the dying cell stimulation of living tumor cell growth was enhanced by the addition of SHH signaling agonists and inhibited by SHH signaling antagonists. SHH agonists also enhanced reporter cell growth in the absence of irradiated feeder cells, suggesting this mechanism plays a role in feeder cell growth stimulation. Given these results, we conclude that SHH signaling activation plays an important role during tumor repopulation after radiotherapy.

Duke Scholars

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

6

Start / End Page

e65032

Location

United States

Related Subject Headings

  • Zinc Finger Protein GLI1
  • X-Rays
  • Tumor Cells, Cultured
  • Transcription Factors
  • Signal Transduction
  • Radiotherapy Dosage
  • RNA, Small Interfering
  • Pancreatic Neoplasms
  • Luciferases
  • Humans
 

Citation

APA
Chicago
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Ma, J., Tian, L., Cheng, J., Chen, Z., Xu, B., Wang, L., … Huang, Q. (2013). Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy. PLoS One, 8(6), e65032. https://doi.org/10.1371/journal.pone.0065032
Ma, Jingjing, Ling Tian, Jin Cheng, Zhiwei Chen, Bing Xu, Liwei Wang, Chuanyuan Li, and Qian Huang. “Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy.PLoS One 8, no. 6 (2013): e65032. https://doi.org/10.1371/journal.pone.0065032.
Ma J, Tian L, Cheng J, Chen Z, Xu B, Wang L, et al. Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy. PLoS One. 2013;8(6):e65032.
Ma, Jingjing, et al. “Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy.PLoS One, vol. 8, no. 6, 2013, p. e65032. Pubmed, doi:10.1371/journal.pone.0065032.
Ma J, Tian L, Cheng J, Chen Z, Xu B, Wang L, Li C, Huang Q. Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy. PLoS One. 2013;8(6):e65032.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

6

Start / End Page

e65032

Location

United States

Related Subject Headings

  • Zinc Finger Protein GLI1
  • X-Rays
  • Tumor Cells, Cultured
  • Transcription Factors
  • Signal Transduction
  • Radiotherapy Dosage
  • RNA, Small Interfering
  • Pancreatic Neoplasms
  • Luciferases
  • Humans