RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

Journal Academic Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

Full Text

Duke Authors

Cited Authors

  • Perez-Pinera, P; Kocak, DD; Vockley, CM; Adler, AF; Kabadi, AM; Polstein, LR; Thakore, PI; Glass, KA; Ousterout, DG; Leong, KW; Guilak, F; Crawford, GE; Reddy, TE; Gersbach, CA

Published Date

  • October 2013

Published In

Volume / Issue

  • 10 / 10

Start / End Page

  • 973 - 976

PubMed ID

  • 23892895

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

Digital Object Identifier (DOI)

  • 10.1038/nmeth.2600

Language

  • eng

Citation Source

  • PubMed