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Are C-reactive protein associated genetic variants associated with serum levels and retinal markers of microvascular pathology in Asian populations from Singapore?

Publication ,  Journal Article
Dorajoo, R; Li, R; Ikram, MK; Liu, J; Froguel, P; Lee, J; Sim, X; Ong, RT-H; Tay, WT; Peng, C; Young, TL; Blakemore, AIF; Cheng, CY; Aung, T ...
Published in: PLoS One
2013

INTRODUCTION: C-reactive protein (CRP) levels are associated with cardiovascular disease and systemic inflammation. We assessed whether CRP-associated loci were associated with serum CRP and retinal markers of microvascular disease, in Asian populations. METHODS: Genome-wide association analysis (GWAS) for serum CRP was performed in East-Asian Chinese (N = 2,434) and Malays (N = 2,542) and South-Asian Indians (N = 2,538) from Singapore. Leveraging on GWAS data, we assessed, in silico, association levels among the Singaporean datasets for 22 recently identified CRP-associated loci. At loci where directional inconsistencies were observed, quantification of inter-ethnic linkage disequilibrium (LD) difference was determined. Next, we assessed association for a variant at CRP and retinal vessel traits [central retinal artery equivalent (CRAE) and central retinal vein equivalent (CRVE)] in a total of 24,132 subjects of East-Asian, South-Asian and European ancestry. RESULTS: Serum CRP was associated with SNPs in/near APOE, CRP, HNF1A and LEPR (p-values ≤4.7×10(-8)) after meta-analysis of Singaporean populations. Using a candidate-SNP approach, we further replicated SNPs at 4 additional loci that had been recently identified to be associated with serum CRP (IL6R, GCKR, IL6 and IL1F10) (p-values ≤0.009), in the Singaporean datasets. SNPs from these 8 loci explained 4.05% of variance in serum CRP. Two SNPs (rs2847281 and rs6901250) were detected to be significant (p-value ≤0.036) but with opposite effect directions in the Singaporean populations as compared to original European studies. At these loci we did not detect significant inter-population LD differences. We further did not observe a significant association between CRP variant and CRVE or CRAE levels after meta-analysis of all Singaporean and European datasets (p-value >0.058). CONCLUSIONS: Common variants associated with serum CRP, first detected in primarily European studies, are also associated with CRP levels in East-Asian and South-Asian populations. We did not find a causal link between CRP and retinal measures of microvascular disease.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

7

Start / End Page

e67650

Location

United States

Related Subject Headings

  • White People
  • Singapore
  • Retinal Vein
  • Retinal Artery
  • Receptors, Leptin
  • Polymorphism, Single Nucleotide
  • Middle Aged
  • Microvessels
  • Male
  • Linkage Disequilibrium
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dorajoo, R., Li, R., Ikram, M. K., Liu, J., Froguel, P., Lee, J., … Friedlander, Y. (2013). Are C-reactive protein associated genetic variants associated with serum levels and retinal markers of microvascular pathology in Asian populations from Singapore? PLoS One, 8(7), e67650. https://doi.org/10.1371/journal.pone.0067650
Dorajoo, Rajkumar, Ruoying Li, Mohammad Kamran Ikram, Jianjun Liu, Philippe Froguel, Jeannette Lee, Xueling Sim, et al. “Are C-reactive protein associated genetic variants associated with serum levels and retinal markers of microvascular pathology in Asian populations from Singapore?PLoS One 8, no. 7 (2013): e67650. https://doi.org/10.1371/journal.pone.0067650.
Dorajoo, Rajkumar, et al. “Are C-reactive protein associated genetic variants associated with serum levels and retinal markers of microvascular pathology in Asian populations from Singapore?PLoS One, vol. 8, no. 7, 2013, p. e67650. Pubmed, doi:10.1371/journal.pone.0067650.
Dorajoo R, Li R, Ikram MK, Liu J, Froguel P, Lee J, Sim X, Ong RT-H, Tay WT, Peng C, Young TL, Blakemore AIF, Cheng CY, Aung T, Mitchell P, Wang JJ, Klaver CC, Boerwinkle E, Klein R, Siscovick DS, Jensen RA, Gudnason V, Smith AV, Teo YY, Wong TY, Tai E-S, Heng C-K, Friedlander Y. Are C-reactive protein associated genetic variants associated with serum levels and retinal markers of microvascular pathology in Asian populations from Singapore? PLoS One. 2013;8(7):e67650.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

7

Start / End Page

e67650

Location

United States

Related Subject Headings

  • White People
  • Singapore
  • Retinal Vein
  • Retinal Artery
  • Receptors, Leptin
  • Polymorphism, Single Nucleotide
  • Middle Aged
  • Microvessels
  • Male
  • Linkage Disequilibrium