Skip to main content
Journal cover image

A single-molecule stretching method for lateral and normal AFM lever calibration.

Publication ,  Journal Article
Dendzik, M; Kulik, A; Benedetti, F; Marszalek, PE; Dietler, G
Published in: Nanotechnology
September 2013

A novel method for quantitative lateral force measurement (LFM) calibration has been developed. Using a single-molecule spectroscopy approach it is possible to calibrate the AFM levers for both lateral and normal spring constants with a single image scan. Moreover, our method does not involve tip modifications. Dextran molecules were chosen for testing our calibration procedure due to their characteristic plateau feature in the force-elongation curve which enables an easy identification of single-molecule stretching events. Using a non-standard (tilted) geometry of AFM scanning, it is possible to observe different components of the stretching force on both normal and lateral force signals. These signals can be further compared to the values obtained by standard (normal) spectroscopic measurements. The values of the normal spring constant obtained with our method are in good agreement with the results obtained from the method exploiting the energy equipartition theorem. The statistical analysis shows that the approach proposed in our paper gives reproducible results of the lateral sensitivity with a relative standard deviation less than 15%.

Duke Scholars

Published In

Nanotechnology

DOI

EISSN

1361-6528

ISSN

0957-4484

Publication Date

September 2013

Volume

24

Issue

36

Start / End Page

365703

Related Subject Headings

  • Nanoscience & Nanotechnology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dendzik, M., Kulik, A., Benedetti, F., Marszalek, P. E., & Dietler, G. (2013). A single-molecule stretching method for lateral and normal AFM lever calibration. Nanotechnology, 24(36), 365703. https://doi.org/10.1088/0957-4484/24/36/365703
Dendzik, Maciej, Andrzej Kulik, Fabrizio Benedetti, Piotr E. Marszalek, and Giovanni Dietler. “A single-molecule stretching method for lateral and normal AFM lever calibration.Nanotechnology 24, no. 36 (September 2013): 365703. https://doi.org/10.1088/0957-4484/24/36/365703.
Dendzik M, Kulik A, Benedetti F, Marszalek PE, Dietler G. A single-molecule stretching method for lateral and normal AFM lever calibration. Nanotechnology. 2013 Sep;24(36):365703.
Dendzik, Maciej, et al. “A single-molecule stretching method for lateral and normal AFM lever calibration.Nanotechnology, vol. 24, no. 36, Sept. 2013, p. 365703. Epmc, doi:10.1088/0957-4484/24/36/365703.
Dendzik M, Kulik A, Benedetti F, Marszalek PE, Dietler G. A single-molecule stretching method for lateral and normal AFM lever calibration. Nanotechnology. 2013 Sep;24(36):365703.
Journal cover image

Published In

Nanotechnology

DOI

EISSN

1361-6528

ISSN

0957-4484

Publication Date

September 2013

Volume

24

Issue

36

Start / End Page

365703

Related Subject Headings

  • Nanoscience & Nanotechnology