Characterization of human immunodeficiency virus type 1 in saliva and blood plasma by V3-specific heteroduplex tracking assay and genotype analyses.

Journal Article (Journal Article)

The gp120 V3-encoding region of human immunodeficiency virus type 1 (HIV-1) RNA derived from the saliva and blood plasma of 11 individuals was characterized by heteroduplex tracking assay and sequence analyses. R5-like viral variants were identified in both fluids of all subjects. X4-like variants were detected in the plasma and/or saliva of three subjects, indicating that X4-like variants are not excluded from the saliva compartment. Viral subpopulations were similar in both fluids of most subjects, suggesting that HIV-1 in oral fluids and blood may stem from a common source. These findings raise the possibility of using saliva as a noninvasive fluid for evaluating and monitoring viral evolution in infected persons.

Full Text

Duke Authors

Cited Authors

  • Freel, SA; Williams, JM; Nelson, JA; Patton, LL; Fiscus, SA; Swanstrom, R; Shugars, DC

Published Date

  • May 2001

Published In

Volume / Issue

  • 75 / 10

Start / End Page

  • 4936 - 4940

PubMed ID

  • 11312368

Pubmed Central ID

  • PMC114251

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.75.10.4936-4940.2001


  • eng