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Enabling hyperpolarized (129) Xe MR spectroscopy and imaging of pulmonary gas transfer to the red blood cells in transgenic mice expressing human hemoglobin.

Publication ,  Journal Article
Freeman, MS; Cleveland, ZI; Qi, Y; Driehuys, B
Published in: Magn Reson Med
November 2013

PURPOSE: Hyperpolarized (HP) (129) Xe gas in the alveoli can be detected separately from (129) Xe dissolved in pulmonary barrier tissues (blood plasma and parenchyma) and red blood cells (RBCs) of humans, allowing this isotope to probe impaired gas uptake. Unfortunately, mice, which are favored as lung disease models, do not display a unique RBC resonance, thus limiting the preclinical utility of (129) Xe MR. Here we overcome this limitation using a commercially available strain of transgenic mice that exclusively expresses human hemoglobin. METHODS: Dynamic HP (129) Xe MR spectroscopy, and three-dimensional radial MRI of gaseous and dissolved (129) Xe were performed in both wild-type (C57BL/6) and transgenic mice. RESULTS: Unlike wild-type animals, transgenic mice displayed two dissolved (129) Xe NMR peaks at 198 and 217 ppm, corresponding to (129) Xe dissolved in barrier tissues and RBCs, respectively. Moreover, signal from these resonances could be imaged separately, using a 1-point variant of the Dixon technique. CONCLUSION: It is now possible to examine the dynamics and spatial distribution of pulmonary gas uptake by the RBCs of mice using HP (129) Xe MR spectroscopy and imaging. When combined with ventilation imaging, this ability will enable translational "mouse-to-human" studies of impaired gas exchange in a variety of pulmonary diseases.

Duke Scholars

Published In

Magn Reson Med

DOI

EISSN

1522-2594

Publication Date

November 2013

Volume

70

Issue

5

Start / End Page

1192 / 1199

Location

United States

Related Subject Headings

  • Xenon Isotopes
  • Tissue Distribution
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Radiopharmaceuticals
  • Pulmonary Gas Exchange
  • Nuclear Medicine & Medical Imaging
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
 

Citation

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Freeman, M. S., Cleveland, Z. I., Qi, Y., & Driehuys, B. (2013). Enabling hyperpolarized (129) Xe MR spectroscopy and imaging of pulmonary gas transfer to the red blood cells in transgenic mice expressing human hemoglobin. Magn Reson Med, 70(5), 1192–1199. https://doi.org/10.1002/mrm.24915
Freeman, Matthew S., Zackary I. Cleveland, Yi Qi, and Bastiaan Driehuys. “Enabling hyperpolarized (129) Xe MR spectroscopy and imaging of pulmonary gas transfer to the red blood cells in transgenic mice expressing human hemoglobin.Magn Reson Med 70, no. 5 (November 2013): 1192–99. https://doi.org/10.1002/mrm.24915.
Freeman, Matthew S., et al. “Enabling hyperpolarized (129) Xe MR spectroscopy and imaging of pulmonary gas transfer to the red blood cells in transgenic mice expressing human hemoglobin.Magn Reson Med, vol. 70, no. 5, Nov. 2013, pp. 1192–99. Pubmed, doi:10.1002/mrm.24915.
Journal cover image

Published In

Magn Reson Med

DOI

EISSN

1522-2594

Publication Date

November 2013

Volume

70

Issue

5

Start / End Page

1192 / 1199

Location

United States

Related Subject Headings

  • Xenon Isotopes
  • Tissue Distribution
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Radiopharmaceuticals
  • Pulmonary Gas Exchange
  • Nuclear Medicine & Medical Imaging
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice