Papillary thyroid carcinomas with and without BRAF V600E mutations are morphologically distinct.


Journal Article

AIMS:  The BRAF V600E mutation resulting in the production of an abnormal BRAF protein has emerged as the most frequent genetic alteration in papillary thyroid carcinomas (PTCs). This study was aimed at identifying distinctive features in tumours with and without the mutation. METHODS AND RESULTS:  Thirty-four mutation-positive and 22 mutation-negative tumours were identified by single-strand conformation polymorphism of the amplified BRAF V600E region in the tumour DNA. Mutation-positive tumours were more common in patients older than 45 years (24/33, P = 0.05), in classic (23/30, P = 0.01), tall cell (4/5) and oncocytic/Warthin-like (2/2) variants of PTC, and in subcapsular sclerosing microcarcinomas (4/4). In contrast, all 12 follicular variants (P < 0.0001) and two diffuse sclerosing variants were negative for the mutation. Mutation-positive tumours displayed infiltrative growth (32/34, P = 0.02), stromal fibrosis (33/34, P < 0.001), psammoma bodies (17/34, P = 0.05), plump eosinophilic tumour cells (22/34, P = 0.01), and classic fully developed nuclear features of PTC (33/34, P = 0.0001). Encapsulation was significantly associated with mutation-negative tumours (15/22, P = 0.02). CONCLUSIONS:  BRAF V600E mutation-positive and negative PTCs are morphologically different. Recognition of their morphology may help in the selection of appropriate tumours for genetic testing.

Full Text

Cited Authors

  • Finkelstein, A; Levy, GH; Hui, P; Prasad, A; Virk, R; Chhieng, DC; Carling, T; Roman, SA; Sosa, JA; Udelsman, R; Theoharis, CG; Prasad, ML

Published Date

  • June 2012

Published In

Volume / Issue

  • 60 / 7

Start / End Page

  • 1052 - 1059

PubMed ID

  • 22335197

Pubmed Central ID

  • 22335197

Electronic International Standard Serial Number (EISSN)

  • 1365-2559

International Standard Serial Number (ISSN)

  • 0309-0167

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2559.2011.04149.x


  • eng