Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species.

Journal Article (Journal Article)

Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have been limited to a few mammalian species. Here we found that a set of four mammalian transcription factor genes used to generate iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell (PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds, fish, and fly, which span 550 million years from a common ancestor. These findings are one of the first to show cross-lineage stem cell-like induction, and to generate pluripotent-like cells for several of these species with in vivo chimeras. We suggest that the stem-cell state may be highly conserved across a wide phylogenetic range. DOI:

Full Text

Duke Authors

Cited Authors

  • Rosselló, RA; Chen, C-C; Dai, R; Howard, JT; Hochgeschwender, U; Jarvis, ED

Published Date

  • September 3, 2013

Published In

Volume / Issue

  • 2 /

Start / End Page

  • e00036 -

PubMed ID

  • 24015354

Pubmed Central ID

  • PMC3762186

International Standard Serial Number (ISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.00036


  • eng

Conference Location

  • England