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Invasion and MMP expression profile in desmoid tumours.

Publication ,  Journal Article
Denys, H; De Wever, O; Nusgens, B; Kong, Y; Sciot, R; Le, A-T; Van Dam, K; Jadidizadeh, A; Tejpar, S; Mareel, M; Alman, B; Cassiman, J-J
Published in: Br J Cancer
April 5, 2004

Desmoid tumours are locally invasive soft tissue tumours in which beta-catenin mediated TCF-dependent transcription is activated. The role of soluble factors secreted by the myofibroblastic desmoid tumour, which could stimulate tumour invasiveness, was investigated. Using collagen gel invasion assays, the presence of factors stimulating invasion in desmoid conditioned media (CM) could be established. Since matrix metalloproteinases (MMPs) have been implicated in the process of tumoral invasion, the expression levels of the MMP family members were evaluated. Quantitative reverse transcription-PCR was used to determine the expression levels of MMP1, MMP2, MMP3, MMP7, MMP11, MMP12, MMP13, MMP14 and the inhibitors TIMP1, TIMP2 and TIMP3. Besides overexpression of MMP7, a known TCF-dependent target gene, a striking upregulation of the expression levels of MMP1, MMP3, MMP11, MMP12 and MMP13 in desmoid tumours, compared to unaffected fibroblasts from the same patients, was found. Treating the CM of desmoids with a synthetic and a physiologic MMP inhibitor reduced the invasion-stimulating capacity of the desmoid CM by approximately 50%. These results suggest the involvement of soluble factors, released by the desmoid cells, in stimulating invasion and implicate the MMPs as facilitators of invasion.

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Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

April 5, 2004

Volume

90

Issue

7

Start / End Page

1443 / 1449

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Protein Array Analysis
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Matrix Metalloproteinases
  • Immunoenzyme Techniques
  • Humans
  • Fibromatosis, Aggressive
  • Culture Media, Conditioned
  • 3211 Oncology and carcinogenesis
 

Citation

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Denys, H., De Wever, O., Nusgens, B., Kong, Y., Sciot, R., Le, A.-T., … Cassiman, J.-J. (2004). Invasion and MMP expression profile in desmoid tumours. Br J Cancer, 90(7), 1443–1449. https://doi.org/10.1038/sj.bjc.6601661
Denys, H., O. De Wever, B. Nusgens, Y. Kong, R. Sciot, A. -. T. Le, K. Van Dam, et al. “Invasion and MMP expression profile in desmoid tumours.Br J Cancer 90, no. 7 (April 5, 2004): 1443–49. https://doi.org/10.1038/sj.bjc.6601661.
Denys H, De Wever O, Nusgens B, Kong Y, Sciot R, Le A-T, et al. Invasion and MMP expression profile in desmoid tumours. Br J Cancer. 2004 Apr 5;90(7):1443–9.
Denys, H., et al. “Invasion and MMP expression profile in desmoid tumours.Br J Cancer, vol. 90, no. 7, Apr. 2004, pp. 1443–49. Pubmed, doi:10.1038/sj.bjc.6601661.
Denys H, De Wever O, Nusgens B, Kong Y, Sciot R, Le A-T, Van Dam K, Jadidizadeh A, Tejpar S, Mareel M, Alman B, Cassiman J-J. Invasion and MMP expression profile in desmoid tumours. Br J Cancer. 2004 Apr 5;90(7):1443–1449.

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

April 5, 2004

Volume

90

Issue

7

Start / End Page

1443 / 1449

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Protein Array Analysis
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Matrix Metalloproteinases
  • Immunoenzyme Techniques
  • Humans
  • Fibromatosis, Aggressive
  • Culture Media, Conditioned
  • 3211 Oncology and carcinogenesis