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A case series of recombinant platelet factor 4 for heparin reversal after cardiopulmonary bypass.

Publication ,  Journal Article
Demma, L; Levy, JH
Published in: Anesth Analg
December 2012

BACKGROUND: Platelet factor 4 (PF4) is released by activated platelets and has a strong affinity for heparin. Recombinant PF4 (rPF4) has been previously considered as an alternative to protamine for heparin reversal. However, it has been demonstrated that antibodies directed against the PF4/heparin moiety are important in the pathophysiologic development of heparin-induced thrombocytopenia, a prothrombotic complication for which cardiac bypass patients are at increased risk. METHODS: We retrospectively analyzed a case series from an open-label, comparative phase I-II study of rPF4 and protamine after cardiac surgery to determine the heparin-reversal activity of different doses of IV rPF4. Sixteen patients received rPF4, and 5 received protamine. Activated clotting time (ACT) was used to monitor heparin reversal, with reversal defined as ACT <150 seconds. Platelets, white blood cells, C3a, C5a, fibrinopeptide A, von Willebrand factor antigen, and prothrombin fragment 1.2 were monitored postoperatively as indicators of coagulation and inflammation. RESULTS: Heparin reversal was successful by 10 minutes after administration of rPF4 as measured by ACT in all 16 patients. Specifically, a dose of 5 mg/kg rPF4 resulted in ACT <150 seconds after 5 minutes in 10 of 10 patients. For both treatment groups, there were no bleeding or thrombotic complications, no clinical thrombocytopenia after day 5, and no deaths within the 30-day study period. CONCLUSIONS: Our case series demonstrates that heparin anticoagulation was effectively reversed by the administration of rPF4 without serious complications. Additional studies are needed to further validate the safety and efficacy of exogenous rPF4 administration.

Duke Scholars

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Published In

Anesth Analg

DOI

EISSN

1526-7598

Publication Date

December 2012

Volume

115

Issue

6

Start / End Page

1273 / 1278

Location

United States

Related Subject Headings

  • Thrombosis
  • Thrombocytopenia
  • Retrospective Studies
  • Recombinant Proteins
  • Protamines
  • Postoperative Hemorrhage
  • Postoperative Complications
  • Platelet Factor 4
  • Platelet Count
  • Middle Aged
 

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Demma, L., & Levy, J. H. (2012). A case series of recombinant platelet factor 4 for heparin reversal after cardiopulmonary bypass. Anesth Analg, 115(6), 1273–1278. https://doi.org/10.1213/ANE.0b013e3182662e1a
Demma, Linda, and Jerrold H. Levy. “A case series of recombinant platelet factor 4 for heparin reversal after cardiopulmonary bypass.Anesth Analg 115, no. 6 (December 2012): 1273–78. https://doi.org/10.1213/ANE.0b013e3182662e1a.
Demma, Linda, and Jerrold H. Levy. “A case series of recombinant platelet factor 4 for heparin reversal after cardiopulmonary bypass.Anesth Analg, vol. 115, no. 6, Dec. 2012, pp. 1273–78. Pubmed, doi:10.1213/ANE.0b013e3182662e1a.

Published In

Anesth Analg

DOI

EISSN

1526-7598

Publication Date

December 2012

Volume

115

Issue

6

Start / End Page

1273 / 1278

Location

United States

Related Subject Headings

  • Thrombosis
  • Thrombocytopenia
  • Retrospective Studies
  • Recombinant Proteins
  • Protamines
  • Postoperative Hemorrhage
  • Postoperative Complications
  • Platelet Factor 4
  • Platelet Count
  • Middle Aged