EBV latent membrane protein 2A induces autoreactive B cell activation and TLR hypersensitivity.

Published

Journal Article

EBV is associated with systemic lupus erythematosus (SLE), but how it might contribute to the etiology is not clear. Since EBV-encoded latent membrane protein 2A (LMP2A) interferes with normal B cell differentiation and function, we sought to determine its effect on B cell tolerance. Mice transgenic for both LMP2A and the Ig transgene 2-12H specific for the ribonucleoprotein Smith (Sm), a target of the immune system in SLE, develop a spontaneous anti-Sm response. LMP2A allows anti-Sm B cells to overcome the regulatory checkpoint at the early preplasma cell stage by a self-Ag-dependent mechanism. LMP2A induces a heightened sensitivity to TLR ligand stimulation, resulting in increased proliferation or Ab-secreting cell differentiation or both. Thus, we propose a model whereby LMP2A induces hypersensitivity to TLR stimulation, leading to activation of anti-Sm B cells through the BCR/TLR pathway. These data further implicate TLRs in the etiology of SLE and suggest a mechanistic link between EBV infection and SLE.

Full Text

Duke Authors

Cited Authors

  • Wang, H; Nicholas, MW; Conway, KL; Sen, P; Diz, R; Tisch, RM; Clarke, SH

Published Date

  • September 1, 2006

Published In

Volume / Issue

  • 177 / 5

Start / End Page

  • 2793 - 2802

PubMed ID

  • 16920914

Pubmed Central ID

  • 16920914

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.177.5.2793

Language

  • eng

Conference Location

  • United States