Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors.

Published

Journal Article

The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice. GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl-/- retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl-/- retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.

Full Text

Cited Authors

  • Akimoto, M; Cheng, H; Zhu, D; Brzezinski, JA; Khanna, R; Filippova, E; Oh, ECT; Jing, Y; Linares, J-L; Brooks, M; Zareparsi, S; Mears, AJ; Hero, A; Glaser, T; Swaroop, A

Published Date

  • March 2006

Published In

Volume / Issue

  • 103 / 10

Start / End Page

  • 3890 - 3895

PubMed ID

  • 16505381

Pubmed Central ID

  • 16505381

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0508214103

Language

  • eng