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Replication and meta-analysis of GWAS identified susceptibility loci in Kawasaki disease confirm the importance of B lymphoid tyrosine kinase (BLK) in disease susceptibility.

Publication ,  Journal Article
Chang, C-J; Kuo, H-C; Chang, J-S; Lee, J-K; Tsai, F-J; Khor, CC; Chang, L-C; Chen, S-P; Ko, T-M; Liu, Y-M; Chen, Y-J; Hong, YM; Jang, GY ...
Published in: PLoS One
2013

The BLK and CD40 loci have been associated with Kawasaki disease (KD) in two genome-wide association studies (GWAS) conducted in a Taiwanese population of Han Chinese ancestry (Taiwanese) and in Japanese cohorts. Here we build on these findings with replication studies of the BLK and CD40 loci in populations of Korean and European descent. The BLK region was significantly associated with KD susceptibility in both populations. Within the BLK gene the rs2736340-located linkage disequilibrium (LD ) comprising the promoter and first intron was strongly associated with KD, with the combined results of Asian studies including Taiwanese, Japanese, and Korean populations (2,539 KD patients and 7,021 controls) providing very compelling evidence of association (rs2736340, OR = 1.498, 1.354-1.657; P = 4.74×10(-31)). We determined the percentage of B cells present in the peripheral blood mononuclear cell (PBMC) population and the expression of BLK in the peripheral blood leukocytes (leukocytes) of KD patients during the acute and convalescent stages. The percentage of B cells in the PBMC population and the expression of BLK in leukocytes were induced in patients in the acute stage of KD. In B cell lines derived from KD patients, and in purified B cells from KD patients obtained during the acute stage, those with the risk allele of rs2736340 expressed significantly lower levels of BLK. These results suggest that peripheral B cells play a pathogenic role during the acute stage of KD. Decreased BLK expression in peripheral blood B cells may alter B cell function and predispose individuals to KD. These associative data suggest a role for B cells during acute KD. Understanding the functional implications may facilitate the development of B cell-mediated therapy for KD.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

8

Start / End Page

e72037

Location

United States

Related Subject Headings

  • src-Family Kinases
  • White People
  • Taiwan
  • Republic of Korea
  • Reproducibility of Results
  • Promoter Regions, Genetic
  • Polymorphism, Single Nucleotide
  • Mucocutaneous Lymph Node Syndrome
  • Linkage Disequilibrium
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Chang, C.-J., Kuo, H.-C., Chang, J.-S., Lee, J.-K., Tsai, F.-J., Khor, C. C., … Lee, Y.-C. (2013). Replication and meta-analysis of GWAS identified susceptibility loci in Kawasaki disease confirm the importance of B lymphoid tyrosine kinase (BLK) in disease susceptibility. PLoS One, 8(8), e72037. https://doi.org/10.1371/journal.pone.0072037
Chang, Chia-Jung, Ho-Chang Kuo, Jeng-Sheng Chang, Jong-Keuk Lee, Fuu-Jen Tsai, Chiea Chuen Khor, Li-Ching Chang, et al. “Replication and meta-analysis of GWAS identified susceptibility loci in Kawasaki disease confirm the importance of B lymphoid tyrosine kinase (BLK) in disease susceptibility.PLoS One 8, no. 8 (2013): e72037. https://doi.org/10.1371/journal.pone.0072037.
Chang C-J, Kuo H-C, Chang J-S, Lee J-K, Tsai F-J, Khor CC, Chang L-C, Chen S-P, Ko T-M, Liu Y-M, Chen Y-J, Hong YM, Jang GY, Hibberd ML, Kuijpers T, Burgner D, Levin M, Burns JC, Davila S, International Kawasaki Disease Genetics Consortium, Korean Kawasaki Disease Genetics Consortium, Taiwan Kawasaki Disease Genetics Consortium, Chen Y-T, Chen C-H, Wu J-Y, Lee Y-C. Replication and meta-analysis of GWAS identified susceptibility loci in Kawasaki disease confirm the importance of B lymphoid tyrosine kinase (BLK) in disease susceptibility. PLoS One. 2013;8(8):e72037.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

8

Start / End Page

e72037

Location

United States

Related Subject Headings

  • src-Family Kinases
  • White People
  • Taiwan
  • Republic of Korea
  • Reproducibility of Results
  • Promoter Regions, Genetic
  • Polymorphism, Single Nucleotide
  • Mucocutaneous Lymph Node Syndrome
  • Linkage Disequilibrium
  • Humans