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Identification of a tissue-specific, C/EBPβ-dependent pathway of differentiation for murine peritoneal macrophages.

Publication ,  Journal Article
Cain, DW; O'Koren, EG; Kan, MJ; Womble, M; Sempowski, GD; Hopper, K; Gunn, MD; Kelsoe, G
Published in: J Immunol
November 1, 2013

Macrophages and dendritic cells (DC) are distributed throughout the body and play important roles in pathogen detection and tissue homeostasis. In tissues, resident macrophages exhibit distinct phenotypes and activities, yet the transcriptional pathways that specify tissue-specific macrophages are largely unknown. We investigated the functions and origins of two peritoneal macrophage populations in mice: small and large peritoneal macrophages (SPM and LPM, respectively). SPM and LPM differ in their ability to phagocytose apoptotic cells, as well as in the production of cytokines in response to LPS. In steady-state conditions, SPM are sustained by circulating precursors, whereas LPM are maintained independently of hematopoiesis; however, both populations are replenished by bone marrow precursors following radiation injury. Transcription factor analysis revealed that SPM and LPM express abundant CCAAT/enhancer binding protein (C/EBP)-β. Cebpb(-/-) mice exhibit elevated numbers of SPM-like cells but lack functional LPM. Alveolar macrophages are also missing in Cebpb(-/-) mice, although macrophage populations in the spleen, kidney, skin, mesenteric lymph nodes, and liver are normal. Adoptive transfer of SPM into Cebpb(-/-) mice results in SPM differentiation into LPM, yet donor SPM do not generate LPM after transfer into C/EBPβ-sufficient mice, suggesting that endogenous LPM inhibit differentiation by SPM. We conclude that C/EBPβ plays an intrinsic, tissue-restricted role in the generation of resident macrophages.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

November 1, 2013

Volume

191

Issue

9

Start / End Page

4665 / 4675

Location

United States

Related Subject Headings

  • Spleen
  • Skin
  • Phagocytosis
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Macrophages, Peritoneal
  • Macrophages, Alveolar
  • Lymph Nodes
  • Liver
 

Citation

APA
Chicago
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Cain, D. W., O’Koren, E. G., Kan, M. J., Womble, M., Sempowski, G. D., Hopper, K., … Kelsoe, G. (2013). Identification of a tissue-specific, C/EBPβ-dependent pathway of differentiation for murine peritoneal macrophages. J Immunol, 191(9), 4665–4675. https://doi.org/10.4049/jimmunol.1300581
Cain, Derek W., Emily G. O’Koren, Matthew J. Kan, Mandy Womble, Gregory D. Sempowski, Kristen Hopper, Michael D. Gunn, and Garnett Kelsoe. “Identification of a tissue-specific, C/EBPβ-dependent pathway of differentiation for murine peritoneal macrophages.J Immunol 191, no. 9 (November 1, 2013): 4665–75. https://doi.org/10.4049/jimmunol.1300581.
Cain DW, O’Koren EG, Kan MJ, Womble M, Sempowski GD, Hopper K, et al. Identification of a tissue-specific, C/EBPβ-dependent pathway of differentiation for murine peritoneal macrophages. J Immunol. 2013 Nov 1;191(9):4665–75.
Cain, Derek W., et al. “Identification of a tissue-specific, C/EBPβ-dependent pathway of differentiation for murine peritoneal macrophages.J Immunol, vol. 191, no. 9, Nov. 2013, pp. 4665–75. Pubmed, doi:10.4049/jimmunol.1300581.
Cain DW, O’Koren EG, Kan MJ, Womble M, Sempowski GD, Hopper K, Gunn MD, Kelsoe G. Identification of a tissue-specific, C/EBPβ-dependent pathway of differentiation for murine peritoneal macrophages. J Immunol. 2013 Nov 1;191(9):4665–4675.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

November 1, 2013

Volume

191

Issue

9

Start / End Page

4665 / 4675

Location

United States

Related Subject Headings

  • Spleen
  • Skin
  • Phagocytosis
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Macrophages, Peritoneal
  • Macrophages, Alveolar
  • Lymph Nodes
  • Liver