Effects of cellular activation on anti-HIV-1 restriction factor expression profile in primary cells.


Journal Article

Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.

Full Text

Duke Authors

Cited Authors

  • Raposo, RAS; Abdel-Mohsen, M; Bilska, M; Montefiori, DC; Nixon, DF; Pillai, SK

Published Date

  • November 2013

Published In

Volume / Issue

  • 87 / 21

Start / End Page

  • 11924 - 11929

PubMed ID

  • 23966394

Pubmed Central ID

  • 23966394

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

Digital Object Identifier (DOI)

  • 10.1128/JVI.02128-13


  • eng

Conference Location

  • United States