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Three-dimensional molecular imaging with photothermal optical coherence tomography.

Publication ,  Journal Article
Skala, MC; Crow, MJ; Wax, A; Izatt, JA
Published in: Methods in molecular biology (Clifton, N.J.)
January 2013

Optical coherence tomography (OCT) is a three-dimensional optical imaging technique that has been successfully implemented in ophthalmology for imaging the human retina, and in studying animal models of disease. OCT can nondestructively visualize structural features in tissue at cellular-level resolution, and can exploit contrast agents to achieve molecular contrast. Photothermal OCT relies on the heat-producing capabilities of antibody-conjugated gold nanoparticles to achieve molecular contrast. A pump laser at the nanoparticle resonance wavelength is used to heat the nanoparticles in the sample, and the resulting changes in the index of refraction around the nanoparticles are detected by phase-sensitive OCT. Lock-in detection of the pump beam amplitude-modulated frequency and the detector frequency allow for high-sensitivity images of molecular targets. This approach is attractive for nondestructive three-dimensional molecular imaging deep (approximately 2 mm) within biological samples. The protocols described here achieve a sensitivity of 14 parts per million (weight/weight) nanoparticles in the sample, which is sufficient to differentiate EGFR (epidermal growth factor receptor)-overexpressing cells from minimally expressing cells in three-dimensional cell constructs.

Duke Scholars

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2013

Volume

1026

Start / End Page

85 / 92

Related Subject Headings

  • Tomography, Optical Coherence
  • Tissue Culture Techniques
  • Molecular Imaging
  • Metal Nanoparticles
  • Immunoconjugates
  • Imaging, Three-Dimensional
  • Humans
  • Hot Temperature
  • Gold
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Skala, M. C., Crow, M. J., Wax, A., & Izatt, J. A. (2013). Three-dimensional molecular imaging with photothermal optical coherence tomography. Methods in Molecular Biology (Clifton, N.J.), 1026, 85–92. https://doi.org/10.1007/978-1-62703-468-5_7
Skala, Melissa C., Matthew J. Crow, Adam Wax, and Joseph A. Izatt. “Three-dimensional molecular imaging with photothermal optical coherence tomography.Methods in Molecular Biology (Clifton, N.J.) 1026 (January 2013): 85–92. https://doi.org/10.1007/978-1-62703-468-5_7.
Skala MC, Crow MJ, Wax A, Izatt JA. Three-dimensional molecular imaging with photothermal optical coherence tomography. Methods in molecular biology (Clifton, NJ). 2013 Jan;1026:85–92.
Skala, Melissa C., et al. “Three-dimensional molecular imaging with photothermal optical coherence tomography.Methods in Molecular Biology (Clifton, N.J.), vol. 1026, Jan. 2013, pp. 85–92. Epmc, doi:10.1007/978-1-62703-468-5_7.
Skala MC, Crow MJ, Wax A, Izatt JA. Three-dimensional molecular imaging with photothermal optical coherence tomography. Methods in molecular biology (Clifton, NJ). 2013 Jan;1026:85–92.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2013

Volume

1026

Start / End Page

85 / 92

Related Subject Headings

  • Tomography, Optical Coherence
  • Tissue Culture Techniques
  • Molecular Imaging
  • Metal Nanoparticles
  • Immunoconjugates
  • Imaging, Three-Dimensional
  • Humans
  • Hot Temperature
  • Gold
  • Developmental Biology