Multilayer microfluidic poly(ethylene glycol) diacrylate hydrogels.

Journal Article (Journal Article)

Development of robust, in vivo like tissues in vitro holds the potential to create regenerative medicine-based therapeutics, provide more physiologically significant preclinical models and supply a pharmacological and toxicological screening platform that reflects in vivo systems in both complexity and function. This protocol describes a simple, robust, multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings, using a combination of soft and photo-lithography. This work has direct applications toward the development of robust, complex, cell-laden hydrogels for in vitro diagnostics and regenerative medicine applications.

Full Text

Duke Authors

Cited Authors

  • Cuchiara, MP; West, JL

Published Date

  • January 2013

Published In

Volume / Issue

  • 949 /

Start / End Page

  • 387 - 401

PubMed ID

  • 23329455

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-134-9_24


  • eng